人类快速延迟整流性钾通道基因与基质金属蛋白酶-9在胃癌组织中的表达及其临床意义

Expression and significance of human ether-a-go-go-related gene protein and matrix metalloproteinase-9 in gastric cancers

目的观察人类快速延迟整流性钾通道基因（HERG1）表达的HERG1蛋白和基质金属蛋白酶（MMP）-9在胃癌组织中的表达和相关性，探讨两者与胃癌预后的关系。方法应用免疫组织化学法[链霉菌抗生物素蛋白-过氧化物酶（SP）法]、反转录-聚合酶链反应（RT-qPCR）技术及Western blot方法检测80例胃癌组织标本及对应正常胃黏膜组织中的HERG1蛋白和MMP-9的表达，并进行回顾性随访。结果免疫组织化学、RT-qPCR、Western blot分析HERG1、MMP-9在胃癌组织中的阳性表达率分别为55%，60%；HERG1蛋白表达与淋巴结转移，远处转移关系密切；MMP-9的表达与胃癌的浸润程度、TNM分期、分化程度、淋巴结转移明显相关（P=0.000），而与性别、肿瘤大小、部位无明显相关。结论HERG1蛋白和MMP-9的过度表达与胃癌的发生发展有关，在肿瘤侵袭、转移过程中起重要作用。

ObjectiveTo investigate the expression of human ether-a-go-go-related gene （HERG1） protein and matrix metalloproteinase-9 （MMP-9） in gastric carcinoma and their correlation with prognosis of gastric carcinoma.MethodsReverse transcription and polymerase chain reaction （PCR） assay and Western blotting were used to detect the expression of HERG1 and MMP-9 in 80 cases of gastric carcinomas and corresponding normal gastric tissues, and the patients were followed up.ResultsThe positive expression rate of HERG1 and MMP-9 was 60% and 55% respectively. There was significant correlation between the positive expression rate of MMP-9 with depth of invasion, TNM stage, differention, lymphatic and distant matastasis. The positive rate of HERG1 was correlated with lymphatic and distant metastasis.ConclusionHERG1 protein and MMP-9 expression in gastric carcinoma appears to be well-characterized indicators of prognosis and metastasis and may play an important role in the tumor invasion and metastasis.