大鼠颈动脉迂曲模型的建立

The model of carotid artery tortuosity in rats

目的通过大鼠自体颈动脉移植建立动脉迂曲模型。方法24只大鼠分为正常对照组、动脉迂曲组和阴性对照组。动脉迂曲组大鼠将自体左侧颈总动脉段（1 cm）端端吻合移植至右侧颈总动脉。术前、术后即刻和术后1周分别测量大鼠血压、颈动脉血流、动脉张力和迂曲指数（TI）。结果本实验大鼠总死亡率7.7%。动脉迂曲组大鼠术后1周发现，右侧颈总动脉出现明显迂曲形态，颈动脉张力由术前（43±13）%降至术后（2±9）%（P=0.000），术后1周增加至（13±8）%。术后大鼠颈动脉迂曲指数（TI）明显增加，由术前接近1，明显增加至移植后的1.22±0.15（P=0.031），1周后进一步增加至1.28±0.34（P=0.027）。正常对照组和阴性对照组迂曲指数在不同时间点的离体和在体状态下无明显改变（1.01±0.004比1.04±0.01）。各组血压、血流速度在术前、术后和术后1周差异均无统计学意义。结论大鼠颈动脉自体移植手术可行，成功率高，术后可成功复制动脉迂曲模型。

ObjectiveArtery tortuosity is a common abnormal vessel morphology, and closely related to the pathogenesis and development of many diseases. However, there is no animal model of artery tortuosity for in vivo studies. This paper aimed to produce an animal model of artery tortuosity by autogenous carotid artery transplantation in rats.Methods24 animals were divided into normal group, artery tortuosity group or negative group. For rats in experimental group, one centimeter of the left common carotid artery was cut and implanted into the right carotid artery by end-to-end anastomosis. Blood pressure, artery tension and tortuosity index were measured pre-operation, post-operation and one week after surgery respectively.ResultsTotal mortality was 7.7%. One week after surgery the right common carotid artery in experimental group appeared tortuosity shape. The axial tension increased from （43±13）% pre-operation, to （2±9）% post-operation （P=0.000）, and then （13±8）% 1 week later. The tortuosity index increased significantly from 1 pre-operation to 1.22±0.15 （P=0.030） post-operation, and then to 1.28±0.34 （P=0.027） a week later. For rats in normal group and negative group, the tortuosity index had no significant change at different time points （1.01±0.004 vs. 1.04±0.01）. There was no significant difference in blood pressure and blood velocity prio-and post-operation for all rats.ConclusionThis paper first time reports artery tortuosity model in rats. Autogenous carotid artery transplantation in rats is feasible, and more success rate. After surgery carotid artery developed tortuosity shape, which provide the necessary means for in vivo study of artery tortuosity.