盐胁迫对罗布麻种子萌发及生理特性的影响

Effects of Salt Stress on Seed Germination and Physiological Characteristics of Apocynum venetum

以引种于内蒙古农业大学农场的巴盟(巴彦淖尔盟)、包头、通辽地区的栽培罗布麻种子为供试材料,研究了0(CK)、100、200、300、400mmol/L Na Cl 5个盐浓度胁迫对罗布麻种子萌发及生理特性的影响,比较其耐盐性,并采用盐害指数、盐害级别与隶属函数法对其耐盐性进行综合评价。结果表明:在0~400mmol/L Na Cl范围内,巴盟、包头、通辽地区罗布麻种子随着盐胁迫程度的增加,发芽率、发芽指数和活力指数显著降低;丙二醛(MDA)含量均随着盐浓度的增加呈上升的趋势,而3个地区材料的超氧化物歧化酶(SOD)、过氧化物酶(POD)、脯氨酸和可溶性糖的含量均随着盐浓度的增加呈现先上升后下降的趋势。同时发现,Na Cl浓度低于200mmol/L时,对罗布麻种子萌发影响不明显;采用盐害指数、盐害级别和模糊数学中的隶属函数法对3个地区罗布麻种子萌发过程的抗盐性强弱进行评估,抗盐性大小表现为:巴盟罗布麻>包头罗布麻>通辽罗布麻。

The effects of different salt levels [0 （CK）, 100, 200, 300 and 400mmol/L NaC1] on physiological characteristics of seed germination of Apocynum venetum were investigated, taking Apocynum venetum seed from Bameng, Baotou, Tongliao, regions, in Inner Mongolia as test material. Salt resistance was comprehensively evaluated with salt damage index, salt damage level and subordinative function at this stage to investigate the response of varieties to salt stress. The results showed： The seed germination rate, germination index and vigor index were all significantly reduced when the salt concentration was in 0-400mmol/L NaC1; the content of malondialdehyde （MDA） in Bameng, Baotou and Tongliao areas showed a upward trend with the increase of salt concentration. However, the contents of supemxide dismutase （SOD）, peroxidase （POD）, proline （Pro） and soluble sugar of all three regions showed a upward trend at first and downward trend later with the increase of salt concentration evenly. At the same time, NaC1 concentration less than 200mmol/L had a little influence on seed germination ofApocynum venetum. Salt resistance of Apocynum venetum seeds from 3 areas were evaluated by salt damage index, salt damage level and adapting subordinate function method in Fuzzy Mathematics. Ability of salt-resistance was： Bameng Apocynum venetum 〉 Baotou Apocynum venetum 〉 Tongliao Apocynum venetum,