雷米普利对病毒性心肌炎后心肌组织自噬水平的影响

Effect of ramipril on myocardial autophagy after viral myocarditis

目的观察雷米普利对病毒性心肌组织自噬水平的影响。方法柯萨奇B3型病毒感染BALB/c小鼠,同步设空白组、模型组、雷米普利组、雷米普利组给予雷米普利2 mg/(kg·d)灌胃28 d,空白组和模型组灌胃同等体积的生理盐水28 d后,观察老鼠的死亡率,取血液上清后测定血清中TNF-α蛋白的表达以及心肌组织测定LC3B和P62的蛋白含量表达。结果感染柯萨奇B3型病毒后的BALB/c小鼠的死亡率变高,精神状态变差,炎症因子TNF-α以及LC3B的蛋白水平均上调,P62蛋白水平下降,而雷米普利可以改变这种因病毒引起的死亡和炎症因子以及自噬水平升高。结论雷米普利可能通过调控病毒性心肌炎的自噬水平和炎症水平,达到改善病毒性心肌炎引起的损伤的目的。

Objective To observe the effect of ramipril on autophagy in myocardial tissue of viral myocarditis. Methods BALB/c mice were infected with Coxsackie B3 virus. A blank group, a model group and a ramipril group were established. The ramipril group was given ramipril 2 mg/（kg·d） for 28 days, while the blank group and the model group were given the same volume of normal saline for 28 days.The mortality was observed, and the expression of TNF-a in the serum and the expression of LC3B in the myocardial tissue, and the expression of P62 protein were determined. Results After being infected with Coxsackie B3 virus, BALB/c mice showed higher mortality and worse mental state. The levels of inflammatory factor TNF-a and protein levels of LC3B were all upregulated, while P62 level decreased. Ramipril changed these changes caused by the virus. Conclusion Ramipril may relieve the damage caused by viral myocarditis through the regulation of viral myocarditis autophagy and inflammation levels.