氧化苦参碱通过促进胰腺星状细胞株中Gli2表达发挥抗胰腺纤维化作用

Anti-pancreatic fibrosis of oxymatrine by promoting expression of transcription factors Gli2 in LTC-14 and PANC-1 pancreatic stellate cells

目的研究氧化苦参碱(OM)对接受TGF-β1刺激的永生化的大鼠胰腺星状细胞(LTC-14)细胞和胰腺导管癌细胞(PANC-1)中Gli2表达的影响。方法取LTC-14细胞和PANC-1细胞,分别分为对照组、TGF-β1处理组、TGF-β1+OM处理组、OM对照组,作用12 h后提取蛋白,通过Western blotting法检测相关蛋白表达。结果与对照组相比,LTC-14细胞接受TGF-β1刺激后Gli2表达显著降低,但是α-SMA、FN和Co L-I的蛋白表达显著上升;与TGF-β1处理组比较,OM预处理后使接受TGF-β1刺激的LTC-14细胞Gli2表达显著升高。与对照组相比,PANC-1细胞接受TGF-β1刺激后Gli2表达显著升高,α-SMA、FN和Co L-I的蛋白表达也显著上升;与TGF-β1处理组比较,OM预处理后使接受TGF-β1刺激的PANC-1细胞Gli2表达显著下降。结论 OM可能通过促进LTC-14细胞中Gli2表达而发挥抗胰腺纤维化作用。

Objective To investigate the effect of oxymatrine（OM） on the expression of Gli2 in LTC-14 cells and PANC-1 cells induced by TGF-β1. Methods LTC-14 cells and PANC-1 cells were divided into four groups：control group, TGF-β1 group, TGF-β1 ＋ OM group, and OM group. The protein was extracted after 12 h, and the expressions of related proteins were detected by Western blot. Results Compared with control group, the expression of Gli2 in LTC-14 cells was significantly decreased induced by TGF-β1, while the expressions of α-SMA, FN, and Co L-I were significantly increased. The expressions of Gli2, FN, Co L-I, and α-SMA were increased significantly induced by TGF-β1 when compared with those of control group in PANC-1 cells, and the expression of Gli2 was inhibited by OM pretreatment in TGF-β1 ＋ OM group compared with TGF-β1 group. OM pretreatment can increase the expression of Gli2 before stimulating with TGF-β1 in TGF-β1 ＋ OM group in comparison with TGF-β1 group. Conclusion OM plays an protected role in pancreatic fibrosis through promoting the expression of Gli2 in LTC-14 cells.