微波辅助降解三七茎叶总皂苷生成人参皂苷Rg_5的工艺研究

Optimization of production of ginenoside Rg_5 by microwave-assistant degradation method of total saponins from stems and leaves of Panax notoginseng

目的通过正交试验联用响应面法优化微波辅助降解三七茎叶总皂苷生成人参皂苷Rg_5的工艺。方法运用微波辅助技术使三七茎叶总皂苷降解生成稀有人参皂苷,HPLC法测定人参皂苷Rg_5含量,以人参皂苷Rg_5降解产率为评价指标,在单因素试验的基础上进行正交试验及响应面试验,考察微波温度、微波功率和微波时间对人参皂苷Rg_5降解产率的影响。结果正交试验与响应面试验中各因素对人参皂苷Rg_5降解产率的影响程度一致,影响次序为微波温度>微波功率>微波时间。正交试验优化得到的最佳工艺条件为微波功率500 W、微波温度150℃,微波时间20 min,在此条件下得到的人参皂苷Rg_5产率为44.76%;响应面法优化得到的最佳工艺条件为微波功率540 W、微波温度153℃和微波时间20 min,在此条件下得到的人参皂苷Rg_5产率为43.07%。结论 2种优化工艺方法各具优势,均对从三七茎叶中制备稀有皂苷具有应用价值。

Objective To optimize the production of ginenoside Rg5 by microwave-assistant degradation method of total saponins from the stems and leaves of Panax notoginseng（PN） by orthogonal design and response surface method. Methods Using microwave-assistant degradation technology to obtain rare ginsenosides from the stems and leaves of PN. The content of ginsenoside Rg5 was determined by HPLC. With the production of ginsenoside Rg5 as the evaluation index, the orthogonal experiment design and response surface method were performed on the basis of single factor experiments to investigate the effects of microwave temperature, microwave power, and microwave time on the degradation yield of ginsenoside Rg5. Results The influence of each factor on the yield of ginsenoside Rg5 was the same by the two methods, the order of which was microwave temperature microwave power microwave time. Results indicated that the optimum conditions were the microwave power of 500 W, the microwave temperature of 150 ℃, and the microwave time of 20 min by orthogonal design, resulting in the yield of ginsenoside Rg5 of 44.76%. The yield of ginsenoside Rg5 was 43.07% when the conditions was optimized by response surface method under microwave power 540 W, temperature 153 ℃,and time 20 min. Conclusion Each of the two methods had its own advantages, all of which are valuable for the preparation of rare saponins from the stems and leaves of PN.