血清白细胞介素21水平与大疱性类天疱疮无相关性

There is no correlation between the serum level of interleukin-21 and bullous pemphigoid

目的 探讨大疱性类天疱疮（BP）患者血清白细胞介素（IL）21水平与抗BP180抗体、嗜酸性粒细胞（EOS）水平、BP疾病面积指数（BPDAI）的相关性。方法 酶联免疫吸附试验检测31例BP患者和31例健康对照血清IL-21水平，并检测患者血清抗BP180抗体滴度，评估13例患者BPDAI，分析IL-21水平与抗BP180抗体滴度、EOS计数及BPDAI的关系。结果 患者组血清IL-21水平［M（P25 ~ P75）］为255.28（156.19 ~ 361.59） ng/L，健康对照组为150.47（72.4 ~ 268.73） ng/L，两组差异无统计学意义（P = 0.14）。BP患者血清IL-21水平与抗BP180 抗体水平［82.98（26.82 ~ 132.92） U/ml］无统计学相关性（rs＝0.18，P = 0.332）。27例有EOS计数的BP患者中，血清IL-21与全血EOS绝对值无统计学相关性（rs＝0.104，P = 0.606）。13例评估BPDAI的患者中，血清IL-21与BPDAI亦无统计学相关性（rs＝-0.233，P = 0.464）。结论 BP患者IL-21水平与抗BP180抗体、EOS水平、BPDAI均不相关。

Objective To investigate the correlations of the serum level of interleukin-21 （IL-21） with anti-BP180 antibodies, peripheral eosinophil level, bullous pemphigoid disease area index （BPDAI）in patients with bullous pemphigoid （BP）. Methods A total of 31 patients with bullous pemphigoid and 31 healthy controls were enrolled into this study. Blood samples were collected from all the subjects, and enzyme-linked immunosorbent assay （ELISA） was performed to detect the serum level of IL-21 and serum titer of anti-BP180 antibodies. BPDAI was assessed among 13 patients, and the correlation of the IL-21 level with anti-BP180 antibody titers, eosinophil count and BPDAI were analyzed. Results No significant difference in the serum level of IL-21 was observed between the patients （M[P25 - P75]： 255.28 [156.19 - 361.59] ng/L） and healthy controls （150.47 [72.4 - 268.73] ng/L, P = 0.14）. There was no correlation between the serum level of IL-21 and serum titer of anti-BP180 antibodies （82.98 [26.82 - 132.92]U/ml, rs = 0.18, P = 0.332）. Among the 27 BP patients who underwent blood cell count, no correlation was found between the serum levels of IL-21 and whole-blood eosinophil counts （rs = 0.104, P = 0.606）. Among the 13 BP patients who underwent assessment of BPDAI, there was no correlation between the serum levels of IL-21 and BPDAI scores （rs = 0.233, P = 0.464）. Conclusion The IL-21 level is uncorrelated with anti-BP180 antibody level, peripheral eosinophil level or BPDAI in patients with BP.