沙棘多糖对扑热息痛诱导的小鼠肝损伤保护作用的研究

Protective effect of Seabucthorn Polysaccharide on acetaminophen-induced mice liver injury

目的:研究沙棘多糖(SP)对扑热息痛(APAP)诱导的小鼠急性肝损伤组织的保护作用和机制。方法:C57BL/6雄性小鼠随机分为6组:空白组(Ctrl),APAP模型组(APAP,350 mg/kg),N-乙酰半胱氨酸组(NAC,150 mg/kg),SP低剂量组(APAP/SP100,100 mg/kg),SP高剂量(APAP/SP200,200 mg/kg),SP对照组(SP200,200 mg/kg),SP连续灌胃30d,30 d后腹腔注射APAP建立急性肝损伤模型,NAC在造模前1 h腹腔注射,16 h后处死小鼠。收集血清检测AST、ALT指标,HE染色检测肝脏组织学变化,实时定量PCR检测肝组织炎症因子,Western blot检测TLR4和p-JNK表达。结果:SP降低了APAP诱导的AST和ALT水平,减轻了肝损伤,抑制了TLR4和p-JNK表达,降低了TNF-α和IL-6的水平。结论:SP通过抑制TLR4-p-JNK通路,减轻了APAP诱导的肝损伤。

Objective：To study the protective effect and mechanism of Seabucthorn Polysaccharide（SP）on acetaminophen-induced acute liver injury in mice. Methods： C57 BL/6 male mice were random divided into six groups： Control group,APAP group（APAP,350 mg/kg）,N-acetylcysteine group（NAC,150 mg/kg）,low dose of SP group（APAP/SP100,100 mg/kg）,high dose of SP group（ APAP/SP200,200 mg/kg）,After SP control group（ SP,200 mg/kg）,SP was continuity administrated for 30 days,after 30 days,acute liver injury model was establish through intraperitoneal injection acetaminophen,NAC administrated 1 h before APAP challenge,mice were sacrificed 16 h after APAP treatment. Serum was collected to test AST ALT index,histological changes in liver were detected by HE staining,RT-PCR was used to measure the inflammatory factors in liver tissue and the expression of TLR4 and p-JNK was measured by Western blot. Results： Pretreatment with SP decreased the level of AST and ALT,alleviated APAP-induced liver injury. Meanwhile,SP suppressed the expression of TLR4 and p-JNK. The levels of TNF-α and IL-6 was decreased by SP pretreatment. Conclusion：SP alleviated APAP-induced liver injury by down regulate TLR4-p-JNK pathway.