气相色谱—质谱法同时定量检测香精中3种禁用物质

Simultaneous determination of three prohibited substances in fragrance compound through gas chromatography-mass spectrometry

建立了基于气相色谱—质谱(GC-MS)同时检测香精中香叶腈、2-戊基-2-环戊烯-1-酮、苄氰3种禁用物质的方法,并对方法进行验证。结果表明:该方法在浓度为0.000 5~0.100 0mg/mL时呈现良好的线性关系,相关系数均大于0.990 0;回收率为90%~116%,相对标准偏差为4.2%~5.4%,重复性好。检出限为0.005μg/mL(S/N≥3),满足痕量分析检测要求,可用于香精中香叶腈、2-戊基-2-环戊烯-1-酮、苄氰的定性定量分析。将该方法应用到薄荷香精样品中香叶腈、2-戊基-2-环戊烯-1-酮和苄氰的检测,未检测到阳性样品。

In this study, a gas chromatography mass spectrometry （GC/MS） method was established for the determination of geranyl nitrile, 2-pentyl 2 cyclopenten-l-one and phenyl cyanide in fragrance compound, and the method was also validated. The method had good linearity （〉0.990 0） with the concentration from 0.000 5 mg/mL to 0.100 0 mg/mL, and low limits of detection （LOD） （〈0.005μg/mL） for these three target compounds. And the recovery was from 90% to 116% with relative standard deviation （RSD） from 4.20/00 to 5.4% at quality control concentration. In a word, the method met the need of trace detection, which can be used to test geranyl nitrile, 2-pentyl-2 cyclopenten-l-one and phenyl cyanide in fragrance compound. When the GC-MS method was applied to detect these three components in different pepppermint fragrance samples, none of them was detected out.