纳米磁珠技术检测肺癌循环肿瘤细胞效果的中试研究

Magnetic nanoparticle method for detecting circulating tumor cells of lung cancer:A pilot study

目的探究纳米磁珠技术在肺癌中的应用价值。方法 (1)将A549和NCI-H1975细胞系分别与识别肽混合,检测肿瘤细胞与识别肽的结合率;取肿瘤细胞加入健康人全血,检测回收率;(2)检测34例疑诊肺癌患者的外周血循环肿瘤细胞(CTC),此后行手术治疗或活检明确病理诊断,将CTC计数结果与病理结果比对。结果 (1)识别肽Pep与A549细胞、H1975细胞的结合率分别为80.0%±6.0%、70.1%±4.8%,回收率为57.3%±7.0%、37.3%±6.1%;(2)CTC在肺癌患者中的总体检出率为71.9%,特异性83.3%,受试者工作特征(ROC)曲线下面积为0.792(P=0.003)。结论纳米磁珠法检测CTC在肺癌诊断中的应用将有广阔的前景。

Objective To explore the diagnostic value of circulating tumor cells（CTC） measured by magnetic nanoparticle method in lung cancer. Methods（1） We measured binding capability of A549 or NCI-H1965 cell lines with recognition peptide and capture efficiency by adding tumor cells into the whole blood of healthy human.（2） We measured CTC of 34 patients suspected with lung cancer, and the counting results of CTC were compared with the following pathological results. Results（1） The binding capability was 80.0%±6.0% for A549 and 70.1%±4.8% for H1957, while the capture efficiency was 57.3%±7.0% for A549 and 37.3%±6.1% for H1975.（2） CTCs were identified in 71.9% of patients with lung cancer. The specificity was 83.3%, and area under receiver operating characteristic（ROC） curve was 0.792（P=0.003）. Conclusion CTC measured by magnetic nanoparticle method has promising application in the diagnosis of lung cancer.