糖复康宁提取物对2型糖尿病大鼠的降糖作用研究

Hypoglycemic effect of Tangfukangning extracts on type 2 diabetes animal model

目的研究中药复方糖复康宁提取物2糖尿病模型大鼠的降血糖作用。方法分别以遗传性ZDF模型大鼠和链脲佐菌素(STZ)联合高糖高脂饲料诱导的SD大鼠为此次研究的2型糖尿病模型动物,ig给予低、高剂量(5.0、10.0 g生药/kg)的糖复康宁提取物,金芪降糖片(455 mg/kg)作为阳性药,连续给药42 d,每周测定空腹血糖、给药第38天测定葡萄糖耐量。结果 ZDF模型结果显示,模型组大鼠血糖值波动幅度保持在10%以内,显著高于对照组(P<0.01);给药42 d后,与模型组比较,各给药组血糖均显著下降(P<0.01),金芪降糖片及糖复康宁提取物低、高剂量组血糖下降率分别为(35.9±8.4)%、(22.1±11.1)%、(50.0±9.1)%;STZ模型结果显示,模型组大鼠血糖一直维持在较高水平(21.1~21.6mmol/L),变化幅度相对稳定,显著高于对照组(P<0.01);给药42 d后,与模型组比较,各给药组血糖均下降,其中糖复康宁提取物低、高剂量组差异显著(P<0.01);金芪降糖片及糖复康宁提取物低、高剂量组血糖下降率分别为(18.6±5.7)%、(21.6±7.2)%、(51.7±20.2)%。糖复康宁提取物低、高2个剂量组血糖曲线下面积(AUC)与模型组比较均显著下降(P<0.05、0.01)。结论中药复方糖复康宁对2种2型糖尿病模型均发挥显著降糖效果。

Objective To study the hypoglycemic effect of Tangfukangning（TFKN） extracts on type 2 diabetes mellitus model. Methods Hereditary ZDF（Zucker diabetic fatty） model rats and SD model rats fed with streptozotocin（STZ） and high-sugar/high-lipid diet were applied as the type 2 diabetes animal model for the study. Two groups of model rats were treated with TFKN extracts at low and high doses（5 and 10 g raw medicine/kg） by ig administration and Jinqi Jiangtang Tablets（455 mg/kg） used as positive control drug, continuous administration for 42 d. The hypoglycemic effects of TFKN extracts on type 2 diabetes animal model were evaluated by determination of the fasting glucose for every week, and glucose tolerance was determinated at the 38 th day of administration. Results The results of ZDF model showed that the fluctuation range of blood glucose in model group was less than 10%, which was significantly higher than that in control group（P〈0.01）. Compared with model group, the blood glucose level of each drug group was significantly decreased after 42 days＇ administration（P〈0.01）. The decreasing percentage of blood glucose in Jinqi Jiangtang Tablets and low and high doses of TFKN extracts groups were（22.1 ± 11.1）%,（35.9 ± 8.4）%, and（50.0± 9.1）%, respectively. The results of STZ model showed that the blood glucose of model group rats remained at a high level（21.1-21.6 mmol/L）, the amplitude of the change was relatively stable and significantly higher than that of the control group（P〈0.01）. Compared with model group, the blood glucose level of each drug group was decreased after 42 days＇ administration, and the difference of the low and high dose group of TFKN extracts was significant（P〈0.01）. The decreasing percentage of blood glucose in Jinqi Jiangtang Tablets and TFKN extracts low and high doses groups were（18.6± 5.7）%,（21.6± 7.2）%, and（51.7± 20.2）%, respectively. The area under the curve（AUC） of blood glucose in the low and high dose group of TFKN extracts was significantly lower than that in model group（P〈0.05 and 0.01）. Conclusion The hypoglycemic effect of TFKN extracts is significant effective in these two type 2 diabetes animal models.