大豆PLMT基因的克隆及在盐、碱胁迫下的表达分析

Cloning of Soybean PLMT Gene and Expression Analysis of It under Saline-Alkaline Stress

为了探明GmPLMT在大豆盐、碱胁迫中的作用及变化趋势,本研究从吉育72号大豆中克隆了GmPLMT基因的cDNA,设置3种不同NaCl、NaOH条件对大豆进行胁迫,分别处理24 h、48 h、72 h、96 h,复水处理24 h,以未处理的大豆材料为对照,测定大豆叶片GmPLMT的相对表达。实验结果表明:成功克隆GmPLMT全长627 bp c DNA序列,包含完整504 bp ORF阅读框;经NaCl、NaOH胁迫处理后,GmPLMT的表达水平均明显增加,经50 mmol/L NaOH胁迫处理96 h并复水处理24 h后,GmPLMT相对表达水平达到最大值0.223 1,是对照的1.13倍。磷脂酰胆碱是植物生长发育的重要代谢物,也是植物细胞膜主要脂质组分。大豆磷脂酰甲基转移酶(GmPLMT)是卵磷脂合成的关键酶,有研究表明PLMT与植物的盐、碱胁迫有关,为大豆GmPLMT功能的深入研究提供理论帮助。

In order to investigate the effect and variation trend of GmPLMT under saline and alkali stress, the cD-NA of GmPLMT gene was cloned from Jiyu No.72 soybean. Three different concentrations of NaCl and NaOH conditions were set to induce soybean for 24 h, 48 h, 72 h and 96 h, respectively, and rehydration for 24 h. The untreated soybean materials were taken as the control to determine the relative expression of GmPLMT in soybean leaves. The results showed that the full-length 627 bp cDNA sequence of GraPLMT was successfully cloned, containing a complete open reading frame （ORF） of 504 bp. The expression level of GmPLMT significantly increased after the treatment of NaCl and NaOH stresses. After 50 mmol/L NaOH stress treatment for 96 h and rehydration treatment for 24 h, the relative expression level of GmPLMT reached the maximum value of 0.2231, which was 1.13 times that of the control. Phosphatidylcholine is an important metabolite in plant growth and development, and also a major lipid component of plant cell membrane. Soybean phosphatidyl methyltransferase （GmPLMT） is a key enzyme in the process of lecithin synthesis, and some studies indicated that PLMT was associated with saline and alkali stress, which could provide a theoretical reference for further research on the function of soybean Gm PLMT.