宫颈癌中Plk1蛋白表达与疾病进展及患者远期生存率的关系

Relationship between Plk1 protein expression and disease progression and long-term survival in cervical cancer patients

目的探讨宫颈癌组织中polo-like kinase 1(Plk 1)蛋白的表达与肿瘤发生发展、远期生存率的关系。方法选取80例宫颈癌组织标本(宫颈癌组)、宫颈上皮瘤组织作为CIN组(40例)、正常宫颈组织标本40例(对照组),收集时间2011年1月-2012年12月,采用免疫组化染色检测3组标本中的Plk1蛋白表达情况,分析Plk1蛋白与宫颈癌发生发展、远期生存率的关系。结果宫颈癌组的Plk1蛋白阳性表达率(73.75%)显著高于CIN组(45.00%)、对照组(12.50%),差异具有统计学意义(P<0.05);宫颈癌组的Plk1蛋白阳性表达与分化程度、国际妇产联盟(FIGO)分期、是否发生淋巴结转移、间质浸润程度、是否发生脉管浸润具有显著的统计学意义(P<0.05);宫颈癌组的Plk1蛋白阳性表达与患者肿瘤直径、年龄的关系无统计学意义(P>0.05);宫颈癌组的Plk1蛋白阳性表达患者的3年生存率76.27%与阴性表达组的85.71%差异无统计学意义(P>0.05);宫颈癌组的Plk1蛋白阳性表达患者的5年生存率40.68%显著低于阴性表达组的66.67%,差异有统计学意义(P<0.05)。结论与CIN组织和正常宫颈组织相比,Plk1蛋白在宫颈癌中表达程度显著上调,并且与肿瘤的进展、患者远期生存密切相关。

Objective To investigate the relationship between the expression of Plk1 protein and the development of tumor and the long-term survival rate in cervical cancer patients. Methods Eighty cases of cervical cancer tissue（cervical cancer group）,40 cases of cervical epithelial tumor（CIN group） and 40 cases of normal cervical tissue（control group） were collected in the pathology department of our hospital from January 2011 to December 2012. The expression of Plk1 protein in three groups of specimens was detected by immunohistochemical staining,and the relationship between the expression of Plk1 protein and the development of cervical cancer and the long-term survival rate was analyzed. Results The positive expression rate of Plk1 in cervical cancer group was73. 75%,which was significantly higher than that in CIN group（45. 00%） and control group（12. 50%）. The difference was statistically significant among the three groups（P〈 0. 05）. The positive expression of Plk1 protein in cervical cancer group was significantly correlated with degree of differentiation,FIGO stage,lymph node metastasis,interstitial infiltration and Lymph vascular invasion（P〈 0. 05）. There was no significant correlation between the positive expression of Plk1 protein in cervical cancer group and tumor diameter and age（P 〉0. 05）. There was no difference in the 3-year survival rate between the patients with Plk1 positive expression（76. 27%） and patients with Plk1 negative expression（85. 71%） in cervical cancer group. The 5-year survival rate of patients with Plk1 positive expression（40. 68%） was significantly lower than that of Plk1 negative expression（66. 67%） in cervical cancer group（P〈 0. 05）. Conclusion Compared with CIN and normal cervical tissues,Plk1 protein is significantly up-regulated in cervical cancer tissues and it is closely related to tumor progression and long-term survival.