摘要
The Ussuri catfish Pseudobagrus ussuriensis is a promising aquaculture fish with sexual dimorphism that males grow 2–3 times faster than females. Therefore, all-male breeding could increase production of this fish, and sex-linked markers would be useful during the breeding process. In this study, a male-associated marker named PuGT54 was isolated in P. ussuriensis from a previously constructed microsatellite library. A total of 185 P. ussuriensis individuals containing 95 females and 90 males were used to test the usage of this maker. And results showed that sexes of all individuals were successfully identified, indicating the high reliability and accuracy of this marker for sex identifi cation. Furthermore, the male-specifi c fragment amplifi ed by this maker was cloned and sequenced, which was 118 base pairs in length. As homologous genes of this fragment was not found through BLASTn, detailed information of the male-specifi c fragment is unknown presently. Nevertheless, this marker would be helpful in conservation, breeding and ecological study of P. ussuriensis.
The Ussuri catfish Pseudobagrus ussuriensis is a promising aquaculture fish with sexual dimorphism that males grow 2–3 times faster than females. Therefore, all-male breeding could increase production of this fish, and sex-linked markers would be useful during the breeding process. In this study, a male-associated marker named PuGT54 was isolated in P. ussuriensis from a previously constructed microsatellite library. A total of 185 P. ussuriensis individuals containing 95 females and 90 males were used to test the usage of this maker. And results showed that sexes of all individuals were successfully identified, indicating the high reliability and accuracy of this marker for sex identifi cation. Furthermore, the male-specifi c fragment amplifi ed by this maker was cloned and sequenced, which was 118 base pairs in length. As homologous genes of this fragment was not found through BLASTn, detailed information of the male-specifi c fragment is unknown presently. Nevertheless, this marker would be helpful in conservation, breeding and ecological study of P. ussuriensis.
作者
ZHU Chuankun
PAN Zhengjun
CHANG Guoliang
DING Huaiyu
WANG Hui
朱传坤;潘正军;王辉;常国亮;丁怀宇(Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huaiyin Normal University;Jiangsu Collaborative Innovation Center of Regional Modern Agriculture & Environmental Protection, Huaiyin Normal University)
基金
Supported by the National Natural Science Foundation of China(No.31602146)
the Scientific Funds of Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection(No.HSXT307)
the Start-up Funds of Scientifi c Research of Huaiyin Normal University(No.31ZCK00)
the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(TAPP)
