摘要
目的研究泽泻总三萜的体内、外抗炎活性。方法建立以脂多糖诱导的BV-2细胞炎症模型,采用ELISA法检测炎症因子IL-6的表达量,观察泽泻总三萜各剂量组的抗炎活性;建立以二甲苯诱导的小鼠耳廓肿胀、大鼠棉球肉芽肿2种炎症模型,以肿胀度为指标再评价泽泻总三萜各剂量组的抗炎活性。结果泽泻总三萜能有效抑制IL-6分泌,减轻小鼠耳廓肿胀及大鼠棉球肉芽肿胀程度。结论泽泻总三萜具有良好的抗炎作用。
Objective: To study the anti-inflammatory activity of total triterpenes from Alisma orientale(TTAO) in vivo and in vitro. Methods: In vitro: the BV-2 microglial cells were stimulated by LPS to establish inflammatory model and the expression of IL-6 in cell supernatant was detected by ELISA to explore the anti-inflammatory activity of TTAO. In vivo: various inflammatory models, including the swelling of ear induced by dimethyl benzene in mice, and granuloma induced by cotton pellet implantation in rat were applied to explore the anti-inflammation of TTAO. Results: TTAO can effectively inhibit the secretion of IL-6 in vitro, and TTAO can effectively reduce ear swelling of mice and the swelling of the granuloma of rats. Conclusion: TTAO has a remarkable anti-inflammatory activity.
作者
林娜
黄锦芳
张雪
罗奋熔
许文
吴水生
LIN Na;HUANG Jinfang;ZHANG Xue;LUO Fenrong;XU Wen;WU Shuisheng(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《福建中医药》
2018年第4期68-69,71,共3页
Fujian Journal of Traditional Chinese Medicine
基金
国家自然科学基金项目(U1205022)
关键词
泽泻
总三萜
IL-6
抗炎活性
Alisma orie ntale
total triterpenes
anti-inflammatory