Fe^(3+)对厌氧产甲烷体系的影响及生物有效性研究

Effect of Fe^(3+) on Anaerobic Methanogenic System and Research of Fe^(3+) Bio-availability

通过间歇实验建立厌氧产甲烷体系,研究不同Fe^(3+)含量对厌氧颗粒污泥消化过程的影响。结果表明,当Fe^(3+)的质量浓度为0~40 mg/L时,甲烷产量随着Fe^(3+)含量增加而增加,加快了微生物对挥发性脂肪酸(VFAs)的利用;高含量Fe^(3+)(质量浓度50~100 mg/L)抑制厌氧产甲烷过程。当Fe^(3+)的质量浓度在40 mg/L时,获得最大甲烷气体体积1.578 L,较对照提高了36.32%。利用Gompertz模型拟合可得,最大产甲烷产率和甲烷潜在产量分别达到11.06m L/h和1.640 L,延滞时间缩短了3.98 h。脱氢酶和F420的质量浓度分别为27.23μg/L和54.85μg/L。且Fe^(3+)的质量浓度在40 mg/L时,污泥对Fe^(3+)水溶态和离子交换态利用率分别提高了90.36%和54.55%,总利用率达25.32%,生物有效性得到提升。

Anaerobic methanogenic system was built by batch experiment,and the effect of different Fe^3＋content on anaerobic granular sludge digestion process was studied.The results showed that,when methane production increased with the increase of Fe^3＋mass concentration from 0-40 mg/L and accelerated the utilization of microorganisms for volatile fatty acids.However,the methanogenesis was inhibited when Fe^3＋content was high（mass concentration was 50-100 mg/L）.The maximum methane production volume was 1.578 L when of mass concentration Fe^3＋was 40 mg/L and increased by 36.32%compared with the control group.The maximum methane production rate and potential methane production increased to 11.06 m L/h and 1.640 L based on a modified-Gompertz model analysis,respectively,and the delay time shortened 3.98 h.The mass concentrations of dehydrogenases and coenzyme F420 reached 27.23μg/L and 54.85μg/L,respectively.When the Fe^3＋mass concentration was 40 mg/L,the utilization rate of water soluble fraction and exchangeable fraction of Fe^3＋increased by 90.36%and 54.55%resulting in a total Fe^3＋utilization reached 25.32%,suggesting that the Fe^3＋bio-availability was enhanced.