雨生红球藻低覆盖度基因组草图分析

The Analysis of the Low Coverage Haematococcus Pluvialis Draft Genome

开展雨生红球藻基因组测序研究,对于解读绿藻起源与进化及生物逆境胁迫响应机制,以及推动雨生红球藻产业发展都具有重要意义。利用Illumina Hiseq 2500对雨生红球藻（Haematococcus pluvialis）进行高通量测序,获得低覆盖度全基因组草图。通过计算k-mer分布预测该基因组草图大小约为547Mb,GC含量为59.2%,为纯合或单倍体。共得到11 059个预测基因,平均基因长度为1 711bp,平均CDS长度为681bp;平均每个基因包含3.2个外显子,外显子的平均长度为353bp。代谢通路分析表明,具有完整的糖酵解、三羧酸循环、磷酸戊糖途径、嘌呤和嘧啶合成等基本代谢通路。

The investigation on the genomic study of Haematococcus pluvialis would be significant to explore the origin and evolution of green algae and the stress responses in Haematococcus pluvialis; and promote the development of Haematococcus pluvialis industry. The low-coverage draft genome of Haematococcus pluvialis was constructed by the Illumina Hiseq 2500 platform. The predicted genome size was approximately 547 Mb,with the GC content of 59. 2% by calculating k-mer distribution. The draft genome contained 11 059 predicted proteincoding genes and the average gene size and CDS were 1 711 bp and 681 bp; every gene contained 3. 2 exons and the size of exon was 353 bp in average. The analysis of metabolic pathway indicated that the low-coverage genome contained whole glycolysis,tricarboxylic acid cycle,phosphopentose,purine and pyrimidine synthesis and other basic metabolism pathway.