蝙蝠葛苏林碱对大鼠离体脑片氧糖剥夺损伤的保护作用

Protective Effect of Daurisoline on Oxygen-glucose Deprivation Injury of Rats' Brain Slices

目的:探讨蝙蝠葛苏林碱(daurisoline,DS)对大鼠脑缺血所致神经细胞损伤的保护作用及作用机制。方法:采用离体大鼠脑片培养,通过氧糖剥夺(oxygen-glucose deprivation,OGD)模拟载体脑缺血损伤模型。脑片经TTC染色,用酶标仪测定吸光度值和乳酸脱氢酶(lactate dehydrogenase,LDH)释放量。结果:TTC染色显示,蝙蝠葛苏林碱各剂量组脑片组织损伤百分率分别下降至(51.37±0.48)%、(33.86±0.27)%、(25.09±0.75)%;LDH释放率分别下降至(53.41±3.89)%、(38.03±3.71)%、(28.03±3.71)%;与OGD组比较,差异有显著统计学意义(P<0.01)。结论:蝙蝠葛苏林碱可以减轻OGD引起的脑片损伤,并呈剂量依赖性。

Objective：To explore the neuroprotective effect of Daurisoline on brain ischemia and mechanism.Methods：The cultured rats＇ brain slices exposed to oxygen-glucose deprivation（ OGD） injury.Brain slices injury was evaluated by MTT staining and the release of lactate dehydrogenase（ LDH） was determined.Results：The TTC staining shown that the injury percentage of rats＇ brain slices with Daurisoline cultured was decreased to（ 51.37 ± 0.48） %,（ 33.86 ± 0.27） % and（ 25.09 ± 0.75） %; The percentage of LDH releasing was decreased to（ 53.41 ± 3.89） %,（ 38.03 ± 3.71） % and（ 28.03 ± 3.71） %,respectively.There were statistical significance to compare with the OGD model group（ P〈0.01）.Conclusion：Daurisoline can exert neuroprotection against OGD-induced injury in cultured brain slices of rats.