匈牙利2个优质杏品系自交亲和性分析

Self-compatibility analysis of two high quality apricot strains introduced from Hungary

为更好地利用国外引进的杏(Armeniaca vulgaris Lam.)种质资源,以北寨红杏(A.vulgaris‘Beizhaihongxing')和金太阳杏(A.vulgaris‘Goldensun')为对照,对从匈牙利引进的优质杏品系Hybrid18/79(A.vulgaris‘18/79')和Hybrid10/10(A.vulgaris‘10/10')的有效花率、自然座果率和自交座果率以及花粉管生长等特性进行研究,同时,通过S-allele-specific PCR扩增程序进行S基因型的分析测定。结果表明:Hybrid18/79、Hybrid10/10和北寨红杏的自交坐果率分别为18.05%,2.27%和1.68%,Hybrid18/79为自交亲和性品系,北寨红杏和Hybrid10/10为自交不亲和品种和品系。与自交亲和的金太阳杏相比,Hybrid10/10自花授粉后花粉管在花柱中生长受到阻塞,至花柱2/3处,花粉管顶端膨大,停止生长,而金太阳杏可以顺利进入胚囊进行受精。Hybrid18/79、Hybrid10/10和北寨红杏的S基因型分别为Su1Su2、S16S16和S30Su1,序列比对发现Hybrid18/79的2个S基因(Su1、Su2)都是新发现的S基因,北寨红杏的一个S基因(Su1)与Hybrid18/79中的一个S基因(Su1)一样,也为新发现的S基因。目前已在GenBank进行了登录,序列登录号为MF685202和MF685203。

In order to make better use of the apricot germplasm resources introduced from Hungary,this study was focused on the effective flower rate,natural fruit setting rate,self-setting fruit rate,pollen tube growth and other characteristics of two high quality apricot strain Hybrid18/79 and Hybrid10/10 introduced from Hungary.Beizhaihongxing and Golden Sun apricot were used as control.The S genotype was assayed and determined by S-allele-specific PCR.The results showed that：The self-pollination fruit setting rates of Hybrid18/79,Hybrid10/10 and Beizhaihongxing were18.05%,2.27% and 1.68% respectively.Hybrid18/79 was a self-compatibility strain.Both Beizhaihongxing and Hybrid10/10 were self-incompatibility cultivars and strains.After self-pollinating,the pollen tube growth of Hybrid 10/10 was blocked in style,and the pollen tube expanded to the top and stopped growing.In comparison,the tube of the self-compatibility cultivar Golden Sun apricot had no growth blocking,which could successfully enter embryo sac for fertilization.The S-genotypes of Hybrid18/79,Hybrid10/10 and Beizhaihongxing were identified as Su1 Su2,S16 S16 and S30 Su1 respectively.The results of sequence alignment showed that Su1 and Su2 were new genes.One S gene（Su1）of Beizhaihongxing apricot was the same as the Su1 in Hybrid18/79.The two discovered genes had been registered in GenBank with accession numbers MF685202 and MF685203.