新疆乌鲁木齐市宠物源沙门氏菌耐药性及耐药基因检测

Detection of drug resistance and resistant genes of Salmonellafrom pets in Urumqi,Xinjiang

为了解新疆乌鲁木齐市宠物源沙门氏菌耐药及耐药基因携带情况,对分离的39株宠物源沙门氏菌采用琼脂稀释法进行药敏试验,PCR方法进行PMQR因子、β-内酰胺酶基因、氨基糖苷类基因、四环素类基因、酰胺醇类基因及多肽类基因检测。结果表明:乌鲁木齐市宠物源沙门氏菌对环丙沙星和阿莫西林的耐药率均高达97.4%(38/39),对诺氟沙星、恩诺沙星、氨苄西林、卡那霉素、四环素和氟苯尼考的耐药率均高达100%(39/39),对头孢噻呋、安普霉素、阿米卡星和庆大霉素100%敏感;耐药菌株以8耐为主,其主要耐药谱型为CIP-NOF-ENR-AMLAMP-KANA-TE-FFC;主要检出的β-内酰胺酶基因有blaTEM和blaOXA;主要检出的PMQR因子有qnrS、oqxA、oqxB和aac(6′)-Ib-cr;主要检出的氨基糖苷类基因有aadA2和ant(3″)-Ia;主要检出的四环素类基因有tetB;主要检出的酰胺醇类基因有floR。耐药基因以blaTEM+blaOXA+qnrS+oqxA+oqxB+aac(6′)-Ib-cr+aadA2+ant(3″)-Ia+tetB+floR共存类型为主。因此临床治疗宠物疾病时应尽量少用人用药物特别是人用新药,多使用兽用敏感药物,避免人发病时无药可用事件的发生。

In order to investigate the resistance to antibacterial drugs and the drug-resistant genes of Salmonella isolates from pets in Urumqi,Xinjiang.Thirty-nine Salmonellaisolates＇ antimicrobial susceptibility were tested by agar dilution method.PCR method was used to detect the resistance genes of PMQR,β-lactamase,aminoglycoside,tetracycline,amide alcohol and peptide.The results showed that：The isolates were 97.4%（38/39）resistant to ciprofloxacin and amoxicillin;The isolates were 100% resistant to norfloxacin,enrofloxacin,ampicillin,kanamycin,tetracycline and florfenicol;The isolates were 100%sensitive to ceftiofur,apramycin,amikacin and gentamicin.The main resistance profile was CIP-NOF-ENR-AML-AMP-KANA-TE-FFC.The blaTEMand blaOXA were the mainly detected genes of beta lactamases.The qnrS,oqxA,oqxBand aac（6′）-Ib-cr were the mainly detected factors of PMQR.The aadA2 and ant（3″）-Ia were the mainly detected genes of aminoglycoside.The tetB and floR were mainly detected genes of tetracycline and amide alcohol.The coexistence of multiple genes was blaTEM ＋blaOXA ＋qnrS ＋oqxA ＋oqxB ＋aac（6′）-Ib-cr＋aadA2＋ant（3″）-Ia＋tetB＋floR.Therefore,to avoid no drug available for treatment human illness situation occurs in future,animal specific sensitive medicines should be used to in pets not drugs for human,especially not new drugs.