微藻营养强化对卤虫生长和营养成分的影响

Effects of microalgae on the growth and nutrient enrichment of cultured Artemia

为研究在培育水产经济动物苗种过程中投喂的营养强化的卤虫幼体,以促进苗种生长及提高存活率,本研究以卤虫(Artemiasp.)为研究对象,利用镜检、凯氏定氮和气相色谱等方法,测定了卤虫发育过程中的生物学体长、蛋白质以及EPA(二十碳五烯酸)和DHA(二十二碳六烯酸)含量,并选择采用湛江叉鞭金藻(Dicrateria zhanjiangensis)、亚心形扁藻(Platymonas subcordiformis)和小球藻(Chlorella pyenoidosa)强化营养卤虫幼体(三龄期),检测了投喂24h和48h后卤虫幼体体长、蛋白质、EPA和DHA含量。结果表明:随着卤虫幼体的发育,EPA含量在120h时显著降低;3种微藻能促进卤虫幼体的生长,但不能提高其蛋白质含量;微藻强化48h后,可以提高卤虫幼体EPA含量。上述结果说明微藻营养强化有利于提高卤虫幼体生长和EPA含量,其中以扁藻的强化效果最为显著。

To investigate the effects of nutrients-enriched Artemia nauplii on the growth and survival rate of aquacultural seedlings during cultivation,Artemia is taken as study object in this study.The body length of Artemia,contents of crude proteins as well as EPA and DHA are determined or assayed by microscopy,Kjeldahl determination and gas chromatography methods during the development following cysts hatching.Three species of microalgae,Dicrateria zhanjiangensis,Platymonas subcordiformis and Chlorella pyenoidosa,are used to feed Artemiafrom 3 instar stage.The body length of Artemia,contents of crude proteins as well as EPA and DHA at 24 hand 48 hfollowing feeding tests are determined to evaluate the effects.The results show that：The EPA content of Artemia is reduced at 120 hfollowing hatching.All the 3 species of microalgae are able to promote growth of Artemia rather than increase the contents of proteins.The enrichment with microalgae for 48 his capable of elevating the content of EPA in Artemia.The results suggest that the enrichment of microalgae is beneficial for the Artemia growth and enhancement of EPA contents.Of three microalgae species tested,P.subcordiformis displays the best performance.