浙江茶区茶尺蠖两近缘种的遗传分化及分布

Genetic differentiation and distribution of two sibling species of tea geometrids in tea-growing areas in Zhejiang,eastern China

【目的】为了揭示浙江省茶区灰茶尺蛾Ectropis grisescens和小茶尺蠖Ectropis obliqua两近缘种的分布情况。【方法】利用线粒体细胞色素氧化酶Ⅰ(mt COⅠ)基因作为分子标记,对采自浙江省17个地点茶园的茶尺蠖533个样品进行了鉴定,并分析了灰茶尺蛾和小茶尺蠖在浙江的地理分布情况。【结果】在调查的17个地点中,11个地点的茶尺蠖样品全部为灰茶尺蛾,另外3个地点(安吉、余杭和龙坞)采集的样品全部为小茶尺蠖,其余的3个地点(杭州西湖、杭州临安和杭州富阳)为两近缘种的混发区。【结论】在浙江省茶区,灰茶尺蛾的分布范围较广,小茶尺蠖的分布范围较窄,同时存在两近缘种的混发区。

【Aim 】To reveal the distribution of the sibling species of Ectropis grisescens and Ectropis obliqua in tea-growing areas in Zhejiang,eastern China. 【Methods】A total of 533 specimens of tea geometrids collected from 17 counties in Zhejiang were identified by using mitochondrial cytochrome oxidase（ mt COI） gene as the molecular marker,and the geographical distribution of E. grisescens and E.obliqua in Zhejiang was also analyzed. 【Results 】 The results showed that among the 17 surveyed localities,the tea geometrids from 11 localities in Zhejiang were all identified as E. grisescens,those from other three localities（ Anji,Yuhang,Longwu） were all E. obliqua,and the rest three localities（ Xihu,Lin＇an and Fuyang districts of Hangzhou） were identified as the co-occurrence areas of the two sibling species. 【Conclusion 】 E. grisescens is widely distributed in tea-growing areas in Zhejiang, the distribution of E. obliqua is narrower,and there exist co-occurrence areas of the two sibling species.