苏木对糖尿病大血管病变中SOD、TNF-α、MCP-1的影响

Effect of Caesalpinia Sappan on the Serum SOD, TNF-α, MCP-1 Levels in Rats with Diabetic Macroangiopathy

目的:探讨苏木对糖尿病大血管病变大鼠血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)水平的影响。方法:将24只雄性SD大鼠随机分为4组,每组6只,分别为对照组、模型组、α-硫辛酸组和苏木组。除去对照组,余下的18只大鼠单次腹腔注射STZ50 mg/kg制备糖尿病大鼠模型,对照组予以注射等体积的柠檬酸-柠檬酸钠缓冲液。成功造模后,苏木组给予0.15 g/kg/d的苏木,α-硫辛酸组给予20 mg/kg/d的α-硫辛酸,每日1次给药,连续给药8周。8周末,检测和比较两组大鼠血清中SOD、TNF-α、MCP-1水平的变化。结果:治疗后,苏木组及α-硫辛酸组血清SOD含量均高于模型组(P<0.05),但苏木组和α-硫辛酸组组间比较无统计学差异(P>0.05);苏木组及α-硫辛酸组血清TNF-α、MCP-1含量均显著低于模型组(P<0.05),与对照组没有明显差别(P>0.05)。结论:苏木可提高糖尿病大血管病大鼠血清SOD含量,降低血清TNF-α、MCP-1含量。

Objective： Investigate the effect of Caesalpinia sappan on the levels of serum superoxide dismutase（SOD）, tumor necrosis factor（TNF-α） and monocyte chemokine（MCP-1） in diabetic rats with macrovascular disease. Method： 24 male rats were randomly divided into 4 groups, 6 rats in each group, the control group, the model group, the α-lipoic acid group and the Caesalpinia sappan group, respectively. Except the control group, STZ50 mg/kg was injected intraperitoneally in other three groups. The control group was injected with equal volume of citric acid sodium citrate buffer. After successful molding, the Caesalpinia sappan group was given0.15 g/kg/d of Caesalpinia sappan, α-lipoic acid group was given alipoic acid of 20 mg/kg/d, the drug was given 1 times a day for 8 weeks. After 8 weeks, the changes of SOD, TNF-α, MCP-1 in serum of two groups of rats were detected and compared. Results： After treatment, the contents of serum SOD in Caesalpinia sappan group andα-lipoic acid group were higher than those in model group.（P〈0.05）, there was no statistical difference between two groups yet（P〉0.05）. The level of serum TNF-α and MCP-1 were significantly lower in the Caesalpinia sappan group and α-lipoic acid group than those in the model group（P〈0.05）, but there was no obvious differ-ence comparing with the control group（P〉0.05）. Conclusion： Caesalpinia sappan can increase the content of SOD and decrease the con-tent of TNF-α and MCP-1 in the serum of diabetic rats with macrovascular disease.