海参斑软骨多糖的提取及其体外抗氧化活性分析

Extraction and vitro antioxidant activity analysis of polysaccharide from lumpus Cartilage

以海参斑软骨为原料,经过粉碎、干燥、脱脂、脱色处理。分别用水提法、碱提法、酶法辅助提取以及超声辅助提取四种方式提取多糖,再用乙醇沉淀冻干得到四种多糖(EAP-W、EAP-D、EAP-U、EAP-E)。将四种多糖进行成分分析,用Sevage法,盐酸调等电点法和三氯乙酸法进行除蛋白工艺优化,最后将除蛋白的四种多糖进行体外抗氧化分析。研究结果表明,四种方式提取多糖提取率分别为3.52%、4.16%、6.84%、4.85%。四种多糖总糖含量分别为52.60%、53.19%、54.81%、54.90%。Sevage法对四种多糖蛋白的去除率分别为61.40%、62.78%、65.26%、60.26%,多糖损失率为21.23%、20.18%、19.15%、26.14%。四种多糖均有一定的抗氧化能力,EAP-W抗氧化活性最好,当浓度为5mg/mL时,EAP-W的还原力、对DPPH自由基、ABTS自由基以及羟基自由基的清除率分别为0.212、83.86%、96.26%、99.01%。

In this study,Lumpus Cartilage was obtained as raw material by smashing、drying、skiming and decoloring. Water extraction,alkali extraction,enzyme-assisted extraction and ultrasonic-assisted extraction were used to extract polysaccharides,then four polysaccharides（EAP-W,EAP-D,EAP-U,EAP-E） were obtained by freeze-drying. The four polysaccharides composition were analyzed,sevage method,isoelectric point method and trichloroacetic acid method were used to optimize deproteinization,finally,vitro antioxidant analysis of four deproteinized polysaccharides were carried out. Results showed that,the yields of four polysaccharides were 3.52%,4.16%,6.84% and 4.85% respectively. The total sugar content of the four polysaccharides were 52.60%,53.19%,54.81% and 54.90% respectively. Sevag method of the four polysaccharide protein removal rates were 61.40%,62.78%,65.26% and 60.26%,polysaccharide loss rates were 21.23%,20.18%,19.15% and 26.14%. Four polysaccharides have a certain antioxidant capacity,the antioxidant activity of EAP-W was the best. When the concentration of EAP-W was 5 mg/mL,the scavenging activity,DPPH free radical,ABTS free radical and hydroxyl free radical of EAP-W were 0.212,83.86%,96.26% and 99.01% respectively.