超高效液相法测定饮料中EDTA-2Na含量

Determination of edetate disodium in beverage by UPLC

建立超高效液相色谱法测定饮料中低浓度EDTA-2Na含量的方法。样品经超声处理10分钟,加入氯化铜溶液和抗坏血酸,在波长为254nm,进样量为20μL的条件下,以甲醇:缓冲液(20:80)为流动相,等度洗脱,外标法定量。检出限可达到1mg/L,质量浓度在1mg/L^10mg/L范围内时,R^2>0.999,回收率为96.5%~100.4%,相对标准偏差为2.45~4.06(n=6)。该方法操作简单、快速、灵敏度高,可满足于饮料中低浓度(1mg/L^10mg/L)EDTA-2Na含量的检测。

To establish a method of determination of the content of low concentration EDTA-2Na in beverage by ultra performance liquid chromatograph,the sample was treated by ultrasonic for 10 minutes and then by CuCl_2 solution and ascorbic acid. The analysis conditions were ：wavelength 254 nm, injection volume 20 μL,methanol to buffer solution 20 ：80 as mobile phase,isocratic elution,and external standard method for quantification. The detection limit can reach to 1 mg/L. When the mass concentration is in the range of 1 mg/L to 10 mg/L,R2 〉 0.999,the recovery rate was 96.5% - 100.4%,and RSD was 2.45 - 4.06（n= 6）. The method is simple,rapid and sensitive,and can be applied to the determination of low concentration EDTA-2Na（1 mg/L - 10 mg/L）in the beverages.