复方黄栌口服液对大鼠肝匀浆及肝组织的影响

Effects of compound Cotinus coggygria oral liquid on liver homogenate and liver tissue of rats

目的:观察复方黄栌口服液对四氯化碳致大鼠慢性肝损伤模型肝匀浆中谷草转氨酶(AST)、谷丙转氨酶(ALT)、丙二醛(MDA)、羟脯氨酸(Hyp)、超氧化物歧化酶(SOD)水平及肝组织的影响。方法:取雌雄各半的SD大鼠72只,随机分为空白组,模型组,护肝宁片组(0.675 g/kg),大、中、小剂量复方黄栌口服液组(15、10、5mL/kg)。采用腹腔注射50%四氯化碳橄榄油溶液1 mL/kg制备大鼠慢性肝损伤模型,2次/周,连续8周,同时第5周开始灌胃相对应的药物。末次给药24 h后,大鼠经水合氯醛腹腔注射麻醉后,经下腔静脉采血,检测肝匀浆中AST、ALT、MDA、Hyp、SOD含量,同时取少量肝组织,观察组织病理学变化。结果:大鼠慢性肝损伤模型造模成功。与模型组相比,大、中、小剂量复方黄栌口服液组和护肝宁片组均可显著降低肝匀浆中ALT、AST、MDA水平(P<0.01),均可显著升高肝匀浆中SOD水平(P<0.01),均可显著降低肝匀浆中Hyp水平(P<0.01),均可显著改善模型大鼠肝脏区病理损伤(P<0.01)。结论:复方黄栌口服液对四氯化碳所致的大鼠慢性肝损伤具有较好的保护作用。

AIM： To observe the effects of compound Cotinus coggygria oral liquid on ALT,AST,SOD,MDA,Hyp levels of liver homogenate and liver tissue in rats with chronic liver injury induced by carbon tetrachloride. METHODS： Seventy-two male SD rats,half male and half female,were randomly divided into blank group,model group,Huganing tablet group（ 0. 675 g/kg）,high,medium and low dose of compound Cotinus coggygria oral liquid group（ 15,10,5 mL/kg,respectively）. Rat model of chronic liver injury was induced by intraperitoneal injection of 50% carbon tetrachloride olive oil solution 1 mL/kg,2 times/week for 8 weeks,and assigned drugs were administered at fifth weeks at the same time accordingly. Twenty-four hours after the final administration,rats were anesthetized by intraperitoneal injection of chloral hydrate,and the inferior vena cava blood was collected to detect the levels of AST,ALT,MDA,Hyp,and SOD in liver homogenate. Also,a small amount of liver tissue was collected to observe the pathological changes. RESULTS： Rat model of chronic liver injury model was successfully induced. Compared with the model group,high,medium and low dose of compound Cotinus coggygria oral liquid and Huganning tablet could significantly reduce the ALT,AST and MDA level（ P〈0. 01）,increase SOD level（ P〈0. 01）,lower Hyp levels（ P〈0. 01,P〈0. 05） in liver homogenate,and could significantly improve liver pathological damages of model rats（ P〈0. 01）. CONCLUSION： Compound Cotinus coggygria oral liquid has a better protective effect on chronic liver injury induced by CCl4.