肌层润性膀胱癌中B7-H3的表达及临床意义

Expression of B7-H3 in muscle infiltrating bladder cancer and its clinical significance

目的 探讨肌层浸润性膀胱癌组织中B7-H3的表达及其临床意义.方法 回顾性分析115例接受膀胱癌根治手术治疗的肌肉浸润性膀胱移行细胞癌患者的临床病理资料.采用免疫组织化学染色法检测膀胱癌组织和对应的癌旁组织中B7-H3的表达,分析其与临床病理学特征的关系.结果 B7-H3的阳性表达主要在细胞浆内,呈棕黄色颗粒.B7-H3在所有膀胱癌组织中均有一定的阳性表达,而在癌旁组织中无表达.B7-H3在膀胱癌组织中的阳性高表达率为68.7％.B7-H3的异常表达与远处转移和脉管内瘤栓均相关（均P＜0.05）,而与患者的性别、年龄、肿瘤分期和分级、淋巴结转移和复发无关（均P＞0.05）.生存分析提示,在膀胱癌组织中B7-H3高表达的患者生存时间明显低于B7-H3低表达患者,差异有统计学意义（P＜0.05）.结论 肌层浸润性膀胱癌组织中存在B7-H3表达水平上调,且与膀胱癌远处转移和脉管内瘤栓正相关;B7-H3的表达可能提示膀胱癌患者不良预后.

Objective To investigate the expression of B7-H3 in muscle infiltration bladder transitional cell carcinoma and its clinical significance.Methods The clinical and pathological data of 115 patients with muscle infiltration bladder transitional cell carcinoma who underwent radical surgery were retrospectively analyzed.The expression of B7-H3 in bladder cancer tissues and corresponding paracancerous tissues was detected by immunohistochemical staining,and the relationship between B7-H3 and clinicopathological characteristics was analyzed.Results The positive expression of B7-H3 was mainly in the cytoplasm,presenting brownish yellow granules.B7-H3 had positive expression in all bladder cancer tissues but no expression in paracancerous tissues.The high positive rate of B7-H3 expression in bladder cancer was 68.7%.The abnormal expression of B7-H3 was correlated with the distant metastasis and intravascular tumor thrombi （all P〈0.05）,but not with gender,age,tumor stage and grade,lymph node metastasis and recurrence （all P〉0.05）.The survival analysis result showed that the survival time of the patients with high expression of B7-H3 in the bladder cancer tissues was significantly lower than that of patients with low expression of B7-H3,and the difference was statistically significant （P〈0.05）.Conclusion The expression of B7-H3 in muscle infiltration bladder cancer tissues is up-regulated and is positively associated with the distant metastasis of bladder cancer and intravascular tumor thrombus.The expression of B7-H3 may indicate poor prognosis of the patients with bladder cancer.