桂林市交通相关PM2.5亚急性染毒对孕鼠及胎鼠的影响

Effects of subacute exposure to traffic-related PM2.5 on pregnancy outcome in rats

目的:探讨桂林市交通相关PM2.5对怀孕大鼠及其子代的影响。方法:于2015年5月至2016年12月在桂林市交通要道旁连续采集交通相关PM2.5,配置为不同浓度PM2.5混悬染毒液。将雌性SD大鼠受孕后随机分为阴性对照组(生理盐水)、低剂量组(0.1mg/mL)、高剂量组(2 mg/mL),每组10只,连续全身暴露染毒17d,观察PM2.5对孕鼠及仔鼠的影响。结果:高剂量组死胎数、吸收胎数增多,活胎数减少,窝重、仔鼠出生体重均明显低于阴性对照组和低剂量组,差异有统计学意义(P<0.05)。结论:妊娠期高剂量交通相关PM2.5暴露可导致子代出生体重降低、死胎、吸收胎等不良妊娠结局。

Objective：To investigate the effects of traffic-related PM2.5 on pregnancy outcome in rats.Methods：PM2.5 samples were collected from the main roadside of Guilin from May 2015 to December 2016 and prepared as PM2.5 suspension.30 healthy gravid rats were randomly divided into control group,low-dose group and high-dose group.Rats in the low-dose group and high-dose group were respectively treated with0.1 mg/mL and 2 mg/mL PM2.5 suspension for 17 days,and the controls were given equal volume of saline.The pregnancy outcomes were observed after PM2.5 exposure.Results：The number of death embryos and absorption embryos was increased,and the number of living embryos was decreased in the high-dose group.The litter weight and body weight of the offspring in high-dose group were significantly lower than those in low-dose group and control group（P〈 0.05）.Conclusion：High dose exposure of traffic-related PM2.5 during pregnancy could lead to lower birth weight,stillbirth,and fetal absorption.