含油废水中筛选的两种石油降解菌的鉴定及降解效果

Identification and Degradation Effect of Two Oil Degrading Bacteria Screened from Oily Wastewater

采用9项生理生化试验以及16S r DNA技术对从含油废水中筛选的2株菌L2、L3进行鉴定,并对L2、L3菌株降解石油烃效果进行了研究.结果表明:2种菌株分别为:L2-Ochrobactrum ciceri sp.苍白杆菌和L3-Serratia marcescens sp.粘质沙雷氏菌.2株单菌对石油烃的降解效果非常高效,初始浓度为4 000 mg/L的石油烃,分别加入菌株L2、L3,120 r/min、30℃下振荡培养6 d后,总石油烃降解率分别高达97.37%、98.98%.两种菌在前3 d对石油降解速率较快,随后进入稳定期生长速率平缓直至趋于零.

Two strains screened from oil wastewanter were identified using 9 physiological and biochemical tests,16 s r DNA technology and degradation effect of petroleum hydrocarbons components by two single strain were studied.Results showed that the two strains were： L2-Ochrobactrum ciceri sp.and L3-Serratia marcescens sp.The effect of 2 strains of single bacteria on petroleum hydrocarbon degradation was very efficient,It revealed that petroleum hydrocarbons degradation rate of L4 was up to 97.37% and 98.98% after incubated in a rotary shaker at 30 ℃ and 120 r/min for 6 days and initial concentration of petroleum hydrocarbons was 4 000 mg/L.Two kinds of bacteria on the first 3 days of the oil degradation rate was faster,and then enter the stationary phase,the growth rate was slow until zero.