基于D101C大孔吸附树脂的原人参三醇组皂苷的分离研究

Study on the Isolation of Protopanaxatriol Type Saponins Based on D101CMacroporous Adsorption Resin

原人参三醇组皂苷(简称PPT型皂苷)是人参中主要的两种四环三萜皂苷之一,在人参茎叶中含量相对较高,具有抗疲劳、抗应激等功效.本文以人参茎叶总皂苷为原料,研究了人参茎叶总皂苷浓度、吸附温度、吸附时间对D101C大孔吸附树脂对PPT型皂苷和PPD型皂苷的吸附影响,同时考察了乙醇浓度对D101C大孔吸附树脂解吸PPT型皂苷和PPD型皂苷的影响.实验结果表明:利用D101C大孔吸附树脂在15℃温度下吸附浓度为15 mg/m L的人参茎叶总皂苷14 h,再利用45%的乙醇水溶液于25℃解吸14 h人参茎叶皂苷时,PPT型皂苷的含量由原来的27.2%提高到86.8%,PPT型皂苷:PPD型皂苷由1.1 1提高到185 1,显示出良好的PPT皂苷的分离及提纯效果.

Protopanaxatriol group（ PPT） is one of the two major tetracyclic triterpenoid saponins,responsible for anti-fatigue,anti-stress effects. This report studied the influence on adsorption of total PPT and PPD on D101 C macroporous resin,by factors including concentration of total ginsenosides in stem and leaf,temperature and time of adsorption. In addition,influence of ethanol percentage was evaluated for desorption. Experimental results demonstrates that D101 C microporous adsorption resin can adsorb 15 mg/m L of total ginsenosides at15 ℃ after 14 hours,with PPT content further elevated to 86. 8% from 27. 2% and increased ratio of PPT to PPD（ 185 1 from 1.1 1） by desorption of 45% ethanol at 25 ℃ for 14 hours,indicating a better extraction and purification method of isolation and purification.