还原型谷胱甘肽与系统性红斑狼疮患者炎性因子的相关性

Relativity between reduced glutathione and inflammatory factors in patients with systemic lupus erythematosus

目的探讨还原型谷胱甘肽(GSH)与系统性红斑狼疮(SLE)患者炎性因子的相关性。方法选取2016年1-12月在该院住院和门诊诊治的SLE患者65例,根据疾病活动情况分为SLE活动组和SLE非活动组,另选取46位同期来该院查体的健康体检者作为对照组。检测SLE患者和对照组血清中GSH、高敏C-反应蛋白(hsCRP)、同型半胱氨酸(Hcy)、补体C3、C4水平;四色流式细胞术检测SLE患者和健康对照组外周血CD3^+、CD4^+、CD8^+T淋巴细胞计数。结果 (1)SLE活动组患者血清hsCRP、Hcy水平和CD8^+淋巴细胞计数高于非活动组和对照组,差异均有统计学意义(P<0.05),GSH、补体C3、C4水平和CD4^+淋巴细胞计数低于非活动组和对照组,差异均有统计学意义(P<0.05)。(2)SLE患者血清GSH水平与hsCRP、Hcy及CD8^+淋巴细胞计数呈负相关(r=-0.53、-0.65、-0.51,P<0.05),与补体C3、CD4^+淋巴细胞计数呈正相关(r=0.64、0.62,P<0.05)。结论活动性SLE患者血管内GSH水平显著降低导致氧化还原态失衡,使hsCRP和Hcy水平升高和CD4^+/CD8^+淋巴细胞倒置,形成炎性状态。

Objective To explore the relativity between reduced glutathione and inflammatory factors in patients with systemic lupus erythematosus.Methods Sixty-five SLE patients visited our hospital from January to December 2016 were included in the study.Additionally,46 people coming for healthy examination during the same period were grouped into control.According to disease activity index,sixty-five patients were divided into SLE activity and non-activity group.T lymphocyte subset of RSLE patients and healthy people were detected by four color fluorescence activated cell sorter.The serum levels of GSH,Hcy,hsCPR,complete C3 and C4 of SLE patients and healthy controls were measured.Results（1）The levels of hsCRP,Hcy and CD8~＋T lymphocyte of activity group were significantly higher than non-activity group（P〈0.05）,and the levels of GSH,complement C3,C4,CD4~＋T lymphocyte were significantly lower than non-activity group（P〈0.05）.（2）The levels of GSH was negatively associated with the level of hsCRP,Hcy and CD8~＋T lymphocyte（r=-0.53,-0.65,-0.51,P〈0.05）,but was positively associated with the level of complenent C3,CD8~＋T lymphocyte（r=0.64,0.62,P〈0.05）.Conclusion GSH decreased significantly in activity SLE patients intravascular may Break the balance of redox state,cause the level of hsCRP,Hcy increased and CD4~＋/CD8~＋lymphocytes reversed,and Form an inflammatory state.