HMGA1 mRNA在乳腺癌中的表达及临床价值

Expression and clinical value of HMGA1 mRNA in breast cancer

目的本文旨在探讨乳腺癌组织中高迁移率族蛋白A1(HMGA1)mRNA的检测对预测乳腺癌发生与发展的临床价值。方法采用RT-PCR的方法检测48对乳腺癌样本(癌及癌旁组织)中HMGA1基因的相对表达,并采用多元线性回归分析HMGA1与临床特征的相关性。通过绘制ROC曲线评估HMGA1预测乳腺癌敏感性和特异性。结果相比癌旁组织,乳腺癌组织中HMGA1mRNA的表达上调;HMGA1mRNA相对表达在淋巴结转移、高组织学分级、雌孕激素受体阴性以及HER-2阴性的乳腺癌组织中明显上调;多元线性回归分析显示HMGA1 mRNA表达与淋巴结转移呈正相关(β=2.627,P<0.001);ROC曲线表明,HMGA1mRNA预测乳腺癌的诊断效能较高(敏感性为83.30%,特异性为95.80%,曲线下面积AUC=0.968),cut-off值等于1.048。结论HMGA1mRNA的高表达预示着患者有较大可能发生乳腺癌,且HMGA1mRNA表达越高,乳腺癌患者的预后越差,有望为预测乳腺癌的发生与发展提供一个特异性的分子标志物。

Objective To investigate the clinical value of detection of high mobility group protein A1（HMGA1）mRNA in breast cancer tissue in predicting the occurrence and development of breast cancer.Methods The relative expressions of HMGA1 gene in 48 pairs of breast cancer samples（cancer and adjacent tissues）were detected by RT-PCR,and the correlation between HMGA1 and clinical features was analyzed by multivariate linear regression analysis.The sensitivity and specificity of HMGA1 in predicting breast cancer were evaluated by drawing ROC curves. Results Compared with normal adjacent tissue,the expressions of HMGA1 mRNA were up-regulated in breast cancer tissue.The relative expressions of HMGA1 mRNA were significantly up-regulated in breast cancer tissues with lymph node metastasis,high histological grade,negative progestin receptor and negative HER-2.Multiple linear regression analysis showed that the expression of HMGA1 mRNA was positively correlated with lymph node metastasis（β=2.627,P〈0.001）.ROC curve showed the higher diagnostic efficacy of HMGA1 mRNA in predicting breast cancer（the sensitivity was 83.30%,the specificity was 95.80% and the area under the curve was 0.968）and the cut-off value was 1.048. Conclusion The high expression of HMGA1 mRNA indicates that breast cancer is more likely to occur,and the higher the expression of HMGA1 mRNA is,the worse the prognosis of breast cancer patient is,which is expected to provide a specific molecular marker for predicting the occurrence and development of breast cancer.