陇南成县玉米耕种区纤维素分解菌的筛选及酶活特性的研究

Screening of Cellulose Decomposing Bacteria in Corn Farming Area of Longnan Cheng County And Study on the Activity of Enzyme

为获得高效纤维素降解菌,以期用于陇南成县玉米秸秆资源的再利用.采用CMC-Na刚果红鉴定初筛培养基和发酵复筛培养基从成县玉米耕种区筛选高效纤维素分解菌,并获得了一株活力较高的菌株,经刚果红鉴定透明圈直径可达24.1 mm,同时经16 sRNA分子鉴定,结合16s-r DNA序列系统发育树分析,鉴定为产二素链霉菌.将该菌株接种于不同碳源的培养基中测其酶活,发现该菌株在24 h内可将滤纸几乎完全瓦解,同时该菌的羧甲基纤维素钠酶活力、对滤纸酶活力和玉米秸秆的酶活力分别达到:5.32 U/m L、6.43 U/m L、25.24 U/m L.因此该菌株在后期秸秆资源的再利用方面具有很高的应用价值.

To obtain high-efficiency cellulose degrading bacteria for the reuse of cornstalk resources of Longnan Cheng County. CMC-Na Congo red identify medium of the screening and the fermentation was used to screening cellulolytic bacteria from the corn farming area of Cheng County. A strain with high vitality was obtained. The diameter of the transparent circle of Congo red was24. 1 mm. At the same time,16 sRNA molecular identification combined with 16 s-r DNA sequence phylogenetic tree analysis,identified as（ Streptomyces ambofaciens）. The strain was detected in the medium of different carbon sources. It was found that the strain could almost completely disintegrate filter paper in 24 h. Meanwhile,the enzyme activity of sodium carboxymethyl cellulose,the activity of filter paper enzyme and the enzyme activity of corn straw were 5. 32 U/mL,6. 43 U/mL and 25. 24 U/mL respectively.Therefore,the strain has high application value in the reutilization of straw resources in the later stage.