高效液相色谱法测定葡萄酒中白藜芦醇含量的研究

Determination of Resveratrol in Wine by HPLC

目的:分析高效液相色谱法(HPLC)测定葡萄酒中白藜芦醇含量的方法。方法:采用HPLC法测定葡萄酒中白藜芦醇含量,色谱柱,C_(18)柱;流动相,乙腈水;波长,306 nm。结果:葡萄酒中白藜芦醇线性范围0.227~45.003μg,检测限、定量限分别为0.074 7、0.228μg/mL,平均回收率为97.10%,RSD为2.25%;精密度试验RSD均<2.0%。结论:高效液相色谱法可作为葡萄酒中白藜芦醇含量的测定方法,其操作简单、准确度高,可在临床检验中推广使用。

Objective： To analyze the HPLC method for the determination of resveratrol in wine. Methods： The content of resveratrol in wine was determined by HPLC. Chromatographic column： C18 column, flowing phase： acetonitrile water, wavelength： 306 nm. Results： The linear range of resveratrol in wine was 0.227 - 45.003 μg, and the detection limit and quantitative limit were 0.074 7 and 0.228 μg/mL respectively, the average recovery rate was 97.10%, RSL） was 2.25%, and the precision test RSL） was less than 2%.. Conclusion： HPLC can be used to determine the content of resveratrol in wine.