摘要
丛枝菌根(arbuscular mycorrhizas,AM)真菌属球囊菌门(Glomeromycota),可以与超过80%的陆生植物形成AM共生体,在植物生长、抗逆、群落稳定性及生态系统稳定性均有重要作用。分子生物学技术和方法普遍应用于AM真菌研究,促进了AM真菌生物学、生态学研究的迅速发展。本文综述了应用RT-PCR、巢式PCR、qPCR技术和cDNAAFLP等分子生物学技术,在AM真菌-植物共生机理、AM真菌快速检测、分类鉴定及群落多样性等方面取得的进展,归纳了利用高通量测序技术、RT-PCR、q-PCR和mRNA等技术,在AM真菌对植物养分吸收、植物抗逆蛋白及转运蛋白基因表达调控的分子机理方面取得的进展,并对未来利用分子生物学技术研究植物-AM真菌互作机理进行了展望。
Arbuscular mycorrhizal（AM）fungi are the most diversely symbiotic plant and soil fungi in the natural ecosystem.The symbionts can enhance the resistance of plants to biotic and abiotic stresses.This paper summarizes the research progress of studies on AM fungi-plant symbiosis,including AM fungal detection,classification,identification,and determination of community diversity.The molecular technologies,RT-PCR,nested PCR,qPCR,cDNA-markers,related primer pairs,and fragment size are summarized.The article also analyzes the mechanism of regulation by AM fungi of plant-related genes and proteins,reveals the molecular mechanism of AM fungi interaction with host plants,and provides a look ahead to the application of molecular biology techniques to study plant-AM fungi interaction mechanisms in the future.
作者
张伟珍
李应德
闫智臣
段廷玉
Zhang Wei-zhen;Li Ying-de;Yan Zhi-chen;Duan Ting-yu(State Key Laboratory of Grassland Agro-ecosystems,Key Laboratory of Grassland Livestock Industry Innovation,Ministry of Agriculture and Rural Affairs,College of Pastoral Agriculture Science and Technology,Lanzhou University,Lanzhou 730020,Gansu,Chin)
出处
《草业科学》
CAS
CSCD
2018年第7期1641-1652,共12页
Pratacultural Science
基金
中央高校基本科研业务费(2022016zr0003)
国家绿肥产业技术体系(CARS-22)
国家牧草产业技术体系(CARS-34)
关键词
AM真菌
分子生物学技术
共生机理
鉴定
检测
群落多样性
AM fungi
molecular biology techniques
symbiotic mechanism
identification
detection
community diversity
