乙醛脱氢酶2基因多态性与胃癌患病风险的相关性研究

Correlation of Genetic Polymorphism in ALDH2 with Risk of Gastric Cancer

目的:研究乙醛脱氢酶2(ALDH2)基因多态性与胃癌患病风险的关联性。方法:346例胃癌患者作为病例组,350例同期健康人群作为对照组;采用PCR-RFLP方法对2组人群的ALDH2基因单核苷酸多态性(SNP)位点rs886205、rs441、rs4646778、rs671及rs2839702的基因型进行检测,比较5个位点的最小等位基因频率(MAF)、基因型频率在2组人群中的分布差异,采用多因素Logistic回归分析5个SNP位点的MAF、基因型频率及不同遗传模型下5个SNP位点与胃癌患病的相关性。结果:5个SNP位点中,仅rs671位点等位基因A与增加胃癌的患病风险具有相关性(OR=1.54,95%CI为1.27~1.91,P=0.001);通过比较各位点的基因型频率,rs886205位点的AG基因型、rs441位点的CC基因型、rs4646778的AA基因型与降低胃癌的患病风险相关(P<0.05),而rs671的AA和AG基因型与增加胃癌的患病风险相关(P<0.05);在隐性模型下,rs441的最小等位基因C和rs4646778的最小等位基因A与降低胃癌患病风险具有相关性(P<0.05),而rs671的最小等位基因A在3个遗传模型下都与增高胃癌患病风险显著相关(P<0.05)。结论:ALDH2基因的rs886205、rs441、rs4646778及rs671位点与胃癌的患病风险具有相关性。

Objective： To investigate the association between genetic polymorphism in ALDH2 and risk of gastric cancer. Methods： A total of 346 gastric cancer patients and 350 healthy controls were enrolled in Beijing university of Chinese medicine hospital of sun simiao from June 2013 to June 2016.Blood samples were collected. The genotypes of rs886205,rs441,rs4646778,rs671 and rs2839702 were detected by polymerase chain reaction-restriction fragment length polumorphism（ PCR-RFLP）method,and the association between SNPs with gastric cancer was evaluated by SPSS 21. 0. Results：By comparing the allele frequencies of SNPs,we found the rs671 were significantly associated with an increased risk of gastric cancer（ OR = 1. 54,95% CI,1. 27 - 1. 91,P = 0. 001）. By comparing the genotype frequencies of SNPs,we found the AG genotype of rs886205,CC genotype of rs441,AA genotype of rs4646778 were associated with decreased risk of gastric cancer; in contrast,AA and AG genotypes of rs671 were associated with increased risk of gastric cancer（ P〈0. 05）. In the genetic model analysis,we found that the minor allele ＂ C＂ of rs441 and the minor allele ＂ A＂ of rs4646778 were associated with decreased risk of gastric cancer under recessive model（ P〈0. 05）. The minor allele ＂ A＂ of rs671 was associated with increased risk gastric cancer under dominant,recessive and additive models（ P〈0. 05）. Conclusion： The rs886205,rs441,rs4646778 and rs671 in ALDH2 were associated with risk of gastric cancer.