电化学发光法高灵敏检测左氧氟沙星

Highly Sensitive Electrochemiluminescence Determination of Levofloxacin

使用柠檬酸钠还原氯金酸制备金纳米粒子(AuNPs),将AuNPs/Nafion悬浮液滴在预先处理好的玻碳电极(GCE)上。根据左氧氟沙星对AuNPs/Nafion/GCE修饰电极上的电化学发光信号物质三联吡啶钌有强的放大作用,构建了一种高灵敏检测左氧氟沙星的电化学发光传感器。实验结果表明,在5.0×10^(-12)~1.0×10^(-9)mol·L^(-1)的浓度范围内,电化学发光的强度与其左氧氟沙星的浓度呈良好的线性关系,检出限为3.0×10^(-12)mol·L^(-1)。本方法操作简便、灵敏、稳定、回收率高,可用于左氧氟沙星注射液的快速测定。

Gold nanoparticles （AuNPs） were prepared by the reduction of chloroauric acid with sodium citrate, and AuNPs/Nafion suspension was dripped onto a pretreated glassy carbon electrode （GCE）. An electrochemiluminescence （ECL） sensor for the determination of levofloxacin has been established, based on the enhanced effect of levofloxacin on the electrochemiluminescence of ruthenium bipyridyl on the AuNPs/Nafion/GCE modified electrode. The results showed that the ECL intensity was directly related to the logarithm of levofloxacin concentration in the range of 5.0×10^（-12） - 1.0×10^（-9） mol·L^-1, with a detection limit of 3.0×10^（-12） mol/L. With the advantages of simple,sensitive,stable,high recovery rate,this method could be used for rapid detection of levofloxacin injection.