台湾牛樟芝液态发酵培养条件优化

Optimization of Liquid Fermentation Culture Method for Antrodia Camphoratafrom Taiwan

采用单因素试验和正交试验,研究台湾牛樟芝液态发酵培养的优化条件。单因素试验表明,黄豆饼粉培养基比马铃薯淀粉更有利于樟芝菌种发酵培养;在相同培养条件下,添加K+盐的樟芝生长效果优于Na+盐。正交试验表明,樟芝液态发酵培养的最优培养条件为葡萄糖30g·L^(-1)、黄豆饼粉30g·L^(-1)、磷酸二氢钾1.5g·L^(-1)、牛樟木屑提取液9mL·L^(-1)。

A single factor test and orthogonal design were used to study the optimization conditions of the nitrogen source,inorganic salt,sawdust extract of Antrodia camphor,the addition of VB and other factors in the liquid fermentation culture of Taiwan Antrodia camphorata.Single factor experiments showed that the culture medium with soybean cake powder is more beneficial to the fermentation of Antrodia camphoratathan with potato starch.Under the same feeding amount,the growth effect of Antrodia camphorataadded with K＋salt was better than that of Na＋salt.The orthogonal experiment showed that the optimum culture conditions for liquid fermentation of Antrodia camphorata were glucose 30 g·L^-1,soybean cake powder 30 g·L^-1,potassium dihydrogen phosphate 1.5 g·L^-1,camphor wood scraps extract 9 ml·L^-1.