噬菌体展示技术在药物研发中的应用

Recent advances of phage display techniques for drug discovery

噬菌体展示技术利用丝状噬菌体展示蛋白和多肽,从大量变异体中提取所需性质的多肽或蛋白。通过噬菌体展示库技术筛选并获得的抗体片段或多肽,在诊断和治疗中发挥着重要的作用。本文简要介绍噬菌体展示技术原理,主要结合噬菌体展示技术在单抗开发、抗原微生物疫苗研发及多肽类药物中的应用,综述噬菌体展示技术在对肿瘤、自身免疫等多种人类疾病诊断及治疗中的重要作用。

Phage display technology utilizes filamentous phage display proteins and polypeptides to extract a desired polypeptide or protein from a large number of variants.The antibody fragments screened and obtained by phage display library technology play an important role in disease diagnosis and treatment.This article briefly introduces the principles of phage display technology,summarizes the development of monoclonal antibodies,the development of antigenic microbial vaccines,and the application of peptide drugs.This review highlights the importance of phage display technology in the diagnosis and treatment of various human diseases such as cancer and autoimmune diseases etc.