选择性激光熔化成型技术制备Ti6Al4V对骨髓间充质干细胞生物学行为的影响

Effect of Ti6Al4V prepared by selective laser melting on the biological behavior of bone marrow mesenchymal stem cells

目的:评价选择性激光熔化成型技术制备Ti6Al4V对骨髓间充质干细胞的生物学行为的影响。方法:应用选择性激光熔化成型技术制备Ti6Al4V合金及纯钛材料。培养比格犬的骨髓间充质干细胞,将其接种于两种材料上,计算细胞在两种材料上的相对增殖率及存活率,并根据细胞相对增殖率对细胞毒性进行分级,并计算细胞早晚期凋亡率及总凋亡率。结果:经过72h的复合培养,纯钛的细胞相对增殖率(97.26±3.78)%优于Ti6Al4V合金(91.77±2.65)%,但无统计学差异,两组的毒性分级均为1级,表明两种材料均无细胞毒性;两组间的细胞存活率无统计学差异。各组材料与犬骨髓间充质干细胞共培养48h后,早期细胞凋亡率、晚期细胞凋亡率及总凋亡率Ti6Al4V试件组、纯钛试件组均高于DMEM组,差异均有统计学意义(P<0.05),但Ti6Al4V试件组与纯钛试件组之间无统计学差异(P>0.05)。结论:两种钛材料对细胞均无细胞毒性,都表现了较好的细胞存活率,两种材料间未见对细胞凋亡的差异,相容性良好。

Objective： To evaluate the effect of selective laser melting（SLM） Ti6Al4V on the biological behavior of bone marrow mesenchymal stem cells. Methods： To compare the anti-hemolysis of Ti6Al4V and pure titanium made by SLM.Bone marrow mesenchymal stem cells from Beagle dogs were cultured and inoculated into two materials. The relative proliferation rate and survival rate of cells on the two materials were calculated, and the cytotoxicity was classified according to the relative cell proliferation rate, and the apoptosis rate and the total apoptosis rate in the morning and evening were calculated. Results： RGR of pure titanium was higher than Ti6Al4V when co-cultured 72 h, but there was no difference between two groups on RGR. The grade of cytotoxicity of two specimens was both grade 1, and did not exhibit cytotoxicity.There was no difference between two groups on survival rate. The early phase rate of apoptosis, late phase rate of apoptosis and totle rate of apoptosis of group Ti6Al4V and group pure titanium were both higher than that of group DMEM when co-cultured 48 h（P〈0.05）, and there was no difference between group Ti6Al4V and group pure titanium（P〉0.05）. Conclusion：Both titanium materials have no cytotoxicity to cells, and both of them show good cell survival rate. There is no difference in apoptosis between the two materials, and the compatibility is good.