8种水产药物对长薄鳅幼鱼的急性毒性试验

Study on Acute Toxicity of Eight Kinds of Aquatic Drugs to Elongateloach( Leptobotia elongata)

在水温(22±1)℃、溶氧量(6.7±0.5)mg/L、p H值7.9±0.2的条件下,采用静态方式水生生物急性毒性试验法,研究8种水产药物对长薄鳅幼鱼(1.95±0.24)g的急性毒性,旨在为长薄鳅的幼鱼培育阶段常规药物的合理使用提供参考数据,并为养殖生产提供有效指导。试验结果显示:氟苯尼考、盐酸强力霉素、聚维酮碘、氯胺T、盐酸奎宁、亚甲基蓝、阿维菌素、敌百虫8种水产药物对长薄鳅幼鱼24 h半致死质量浓度分别为1 691.18、606.03、51.49、32.80、35.97、12.80、0.25、1.54 mg/L;48 h半致死质量浓度分别为1 626.97、486.18、45.51、30.98、26.93、11.16、0.18、1.41 mg/L;72 h半致死质量浓度分别为1 575.34、390.03、40.23、27.96、24.10、9.56、0.16、1.16 mg/L;96 h半致死质量浓度分别为1 515.53、359.99、34.23、27.65、20.17、8.63、0.13、1.10 mg/L;其安全质量浓度分别为451.73、93.87、10.67、8.29、4.53、2.55、0.03、0.35 mg/L。其中,阿维菌素对长薄鳅幼鱼高毒,亚甲基蓝和敌百虫对其中毒,氟苯尼考、盐酸强力霉素、聚维酮碘、氯胺T、盐酸奎宁对其低毒。

Under the conditions of water temperature of （22 ± 1 ） ℃ and dissolved oxygen of （6.7 ± 0.5 ） mg/L and pH value of 7.9 ± 0.2, static method aquatic organism acute toxicity test was used to study the effects of 8 aquatic drugs on acute toxicity of juvenile Leptobotia elougata, which each body weight was （1.95 ± 0.24）g. The aims were to provide reference data for the use of conventional drugs during the juvenile culture period and to provide guidance for actual production. The test results showed that the median lethal concentrations of florfenicol, doxycycline hydrochloride, povidone iodine, chloramine T,quinine hydrochloride, methylene blue, abamectin and trichloffon were 1 691.18,606.03,51.49,32.80, 35.97,12.80,0.25,1.54 mg/L for 24 hours, the 1 626.97,486.18,45.51,30.98,26.93,11.16,0.18, 1.41 mg/L for48 hours,the 1 575.34,390.03,40.23,27.96,24. 10,9.56,0. 16,1. 16 mg/L for 72 hours,and the 1 515.53,359.99,34.23,27.65,20. 17,8.63,0. 13,1. 10 mg/L for 96 hours. The safe concentrations of 8 kinds of aquatic drugs were 451.73,93.87,10.67,8.29,4.53,2.55,0.03,0.35 mg/L, respectively. According to the toxicity of toxic substances on fish toxicity evaluation criteria for Leptobotia elongata,avermectin was highly toxic, methylene blue and trichloffon were medium toxic, floffenicol,doxycycline hydrochloride, povidone iodine, chloramine T, and quinine hydrochloride were low toxic.