胃脂肪酶在胃癌中的表达及临床意义

The expression and clinical significance of lipase type F in gastric cancer

目的研究胃脂肪酶(lipase type F,LIPF)在胃癌中的表达,并分析其与临床病理特征及预后的相关性。方法收集胃癌手术切除后的石蜡包埋组织,包括胃癌组织122例和癌旁正常组织120例,整理临床资料及预后随访资料并进行相关性分析;用组织微阵列及免疫组织化学方法检测组织中LIPF的表达。选取5株胃癌细胞系及1株永生化正常胃黏膜细胞系GES1,利用qRTPCR方法检测LIPF mRNA的表达,用Western blotting方法检测各细胞系及8对新鲜配对胃癌组织中LIPF蛋白的表达。结果 LIPF在胃癌组织中的表达率显著低于癌旁正常组织(P<0.001)。LIPF mRNA及蛋白在胃癌细胞系中微弱表达或不表达,在GES1细胞系中高表达(P<0.01)。低表达LIPF胃癌患者5年内生存率显著低于高表达组(P<0.001)。LIPF的表达与胃癌组织的浸润深度、Lauren分型密切相关(P<0.05),与患者年龄、性别、TNM分期、淋巴结转移、远端转移无明显相关性(P>0.05)。结论 LIPF在胃癌中表达下调,其低表达与肿瘤浸润深度、弥漫型胃癌呈正相关,低表达LIPF胃癌组预后较高表达组差,LIPF可以作为胃癌预后潜在的分子标志物。

Objective To investigate the expression of lipase type F（LIPF） and the correlation with clinicopathological parameters and overall survival of patients with gastric cancer. Methods The paraffin-embedded tissues were collected from gastric cancer patients,including 122 cases of gastric cancer tissues and 120 cases of adjacent normal tissues. The association between the expression of LIPF and other clinicopathological parameters was analyzed in gastric cancer. The expression of LIPF in gastric cancer and normal tissues was analyzed by immunohistochemistry and Western blotting. The expressions of LIPF mRNA and protein in gastric cancer cell lines and immortalized gastric epithelial cell line GES1 were measured by RT-PCR and Western blotting,respectively. Results The positive rate of LIPF in gastric cancer tissues was significantly lower than that in adjacent normal tissues（P〉0. 001）. The expressions of LIPF mRNA and protein in gastric cancer cell lines were weak or silent,which were higher in GES1 cells. The 5-year survival rate of patients with low expression of LIPF in gastric cancer was significantly lower than that in patients with high expression of LIPF（P〈0. 001）. The expression of LIPF was related with invasion and Lauren classification of gastric cancer（P〈0. 05）. The expression of LIPF was not related with age,sex,TNM stage,lymph node metastasis and distant metastasis（P〈0. 05）. Conclusion The expression of LIPF in gastric cancer is down-regulated,and low expression of LIPF is significantly correlated with the depth of invasion,diffused type gastric cancer and poor prognosis,which indicates that LIPF is the potential prognostic biomarker in gastric cancer.