摘要
目的:探讨adipophilin促进细胞内脂质蓄积的可能机制,为动脉粥样硬化的防治提供参考。方法:分别通过q PCR、Western blot和油红O染色观察氧化型低密度脂蛋白(ox LDL)处理RAW264.7细胞不同时间后,细胞内Akt、p-Akt和adipophilin的蛋白水平及脂质蓄积情况;并检测PI3K/Akt抑制剂LY294002处理后,上述指标的变化;HEK293细胞过表达adipophilin后,检测Akt的活性;并用免疫共沉淀实验检测adipophilin与Akt之间的相互作用。结果:ox LDL处理细胞后,随着时间的延长,脂滴增多,Akt被活化,adipophilin表达增加,而LY294002处理则可抑制上述变化;过表达adipophilin后,p-Akt水平增高,但adipophilin与Akt之间未见直接相互作用。结论:Adipophilin可通过PI3K/Akt途径促进细胞内脂质蓄积,但可能不是通过直接相互关系发挥作用的。
AIM: To observe the possible mechanism through which adipophilin promotes the accumulation of intracellular lipids,and to provide a reference for controlling atherosclerosis. METHODS: RAW264. 7 cells were incubated with oxidized low-density lipoprotein( ox LDL) for different time. q PCR,Western blot and Oil red O staining were used to observe the mRNA and protein levels of Akt,p-Akt and adipophilin and lipid accumulation. The above indexes were measured after the cells were treated with PI3 K/Akt signaling pathway inhibitor LY294002. The activation of Akt was analyzed in the HEK293 cells over-expressing adipophilin. Co-immunoprecipitation was applied for analysis of protein-protein interaction between adipophilin and Akt. RESULTS: After incubation with ox LDL,the amount of lipid droplets,Akt activity and adipophilin expression increased in the cells with the extension of time( P〈0. 05). Moreover,LY294002 inhibited the above changes. The p-Akt levels increased after adipophilin over-expression. No direct interaction between adipophilin and Akt proteins was observed. CONCLUSION: Adipophilin promotes the accumulation of intracellular lipids through PI3 K/Akt signaling pathway,but possibly not by direct interaction between adipophilin and Akt proteins.
作者
刘清南
戴志兵
袁中华
刘圆月
谭桂煌
徐桂娜
贺旭
LIU Qing-nan;DAI Zhi-bing;YUAN Zhong-hua;LIU Yuan-yue;TAN Gui-huang;XU Gui-na;HE Xu(Yiyang Medical College;Department of Ultrasonography,Yiyang Central Hospital,Yiyang 413000,China;Institute ofCardiovascular Disease,Key Laboratory for Arteriosclerology of Hunan Province,University of South China,Hengyang421001,China.)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2018年第7期1189-1194,共6页
Chinese Journal of Pathophysiology
基金
湖南省教育厅优秀青年科研项目(No.14B183)