摘要
为鲜切蔬菜快速检测的市场化应用提供理论基础,利用大肠杆菌的特异性基因rfbE基因设计LAMP引物,通过对10株菌株进行特异性检测,建立利用环介导等温扩增技术检测鲜切蔬菜中的大肠杆菌O157∶H7的方法,并进行验证检测。结果表明:检测鲜切蔬菜样品特异性为93.3%,准确度为96.5%,检测大肠杆菌灵敏度达1.69CFU/mL。建立的快速检测方法可满足市场化应用的需要。
In order to provide a theoretical basis for market application of rapid detection of fresh-cut vegetables,the loop-mediated isothermal amplification(LAMP)primer was designed with the specific gene rfbEof Escherichia coli,specific detection of 10 strains was conducted to establish the detection method of E.coli O157∶H7 in fresh-cut vegetables by LAMP,then the detection results were verified as well.Results:The specificity of fresh-cut vegetables was 93.3% with the accuracy of 96.5%,and the detecting sensitivity of E.coli was up to 1.69 CFU/mL.The established rapid detection method could meet the requirement of market application.
出处
《贵州农业科学》
CAS
2018年第7期149-151,共3页
Guizhou Agricultural Sciences
基金
贵州省科技计划项目"微生物快速检测技术集成研究与应用示范"[黔科合(2016)支撑2908]
贵州省工业和信息化发展专项资金计划项目"贵州省食品安全生物检测技术中心能力建设"(2017002)
贵阳市人才创新创业资助项目"环介导等温扩增技术快速检测产品开发"[筑人才办合同字(2016)第13号]
关键词
鲜切蔬菜
大肠杆菌
环介导等温扩增
快速检测
农产品质量检测
fresh-cut vegetable; Escherichia coli
loop-mediated isothermal amplification;rapid detection
quality inspection of agricultural products