不同植物半胱氨酸蛋白酶及其基因的生物信息学分析

Bioinformatics Analysis of Cysteine Protease and Its Genes in Different Plants

采用生物信息学方法对GenBank中已经登录的10种植物的半胱氨酸蛋白酶(Cysteine Protease,CP)基因和氨基酸序列进行了解析,对其组成成分、理化性质、翻译后修饰位点、二级结构、亚细胞定位和分子进化等进行了预测和推断。结果表明:10种植物CP的基因全长在1053~1443 bp,编码350~480个氨基酸,在蛋白质N端存在信号肽;10种植物CP都具有酪蛋白激酶Ⅱ磷酸化位点和N端肉豆蔻酰化修饰位点;大多数植物CP具有N端糖基化位点和蛋白激酶C磷酸化位点;二级结构以α螺旋和无规则卷曲为主;根据构建的CP系统进化树,不同科植物具有一定的亲缘关系。

Bioinformatics method was used to analyze the GenBank-registered genes and amino acid sequences of Cysteine Protease（ CP） in 10 kinds of plants,and their compositions,physical and chemical properties,post-translational modification sites,secondary structure,subcellular location and molecular phylogenetic evolution were predicted and inferred. The results demonstrated that the CP genes in ten kinds of plants were 1053 - 1443 bp in overall length,and they encoded 350 - 480 amino acids and had signal peptides at the N-terminal of the protein. The CPs in ten kinds of plants all possessed casein kinase Ⅱ phosphorylation sites and N-terminal myristoylation modification sites. The CPs in most plants had N-terminal glycosylation sites and protein kinase C phosphorylation sites. The secondary structure mainly included alpha helix and random coil. According to the established phylogenetic tree for CP,there were certain genetic relationships among different kinds of plants.