浆细胞瘤转化迁移基因1在结直肠癌患者血浆中的表达与临床意义

Expression and clinical significance of long non-coding RNA PVT1 in plasma of patients with colorectal cancer

目的:检测长链非编码RNA浆细胞瘤转化迁移基因1(plasmacytoma variant translocation gene 1,PVT1)在结直肠癌患者血浆中的表达并探讨其临床意义。方法:收集90例结直肠癌患者术前血浆、30例结直肠腺瘤患者血浆、42例体检健康者(对照组)血浆及29例配对的结直肠癌患者术后2~3周的血浆标本。采用实时荧光定量PCR(qRT-PCR)方法检测上述标本的PVT1表达水平,并对29例结直肠癌患者术前、术后进行比较。分析血浆PVT1表达量与结直肠癌临床病理参数的相关性。采用受试者工作特征曲线(ROC)评估血浆PVT1对结直肠癌的诊断效能。结果:与对照组相比,结直肠腺瘤组、结直肠癌组及TNMⅠ/Ⅱ期结直肠癌组血浆PVT1表达水平均显著升高(P均<0.05),且结直肠癌患者术后表达水平较术前明显降低(P<0.01);结直肠癌患者血浆中PVT1表达水平与年龄、性别及肿瘤的部位、大小、分化程度、TNM分期等无相关性(P>0.05);血浆PVT1单独诊断结直肠癌的效能高于常规肿瘤标志物癌胚抗原;PVT1与癌胚抗原联合检测对结直肠癌及TNMⅠ/Ⅱ期结直肠癌诊断价值均高于PVT1、癌胚抗原单独检测。结论:lncRNA PVT1在结直肠癌及癌前病变患者血浆中表达上调,其可作为结直肠癌临床诊断和术后监测潜在的循环分子标志物。

Objective： To investigate the expression level of long non-coding RNA（ lncRNA） plasmacytoma variant translocation 1 gene（ PVT1） in plasma of patients with colorectal cancer and its clinical significance. Methods： The plasma samples were collected from 90 patients with colorectal cancer,30 patients with colorectal adenoma,42 healthy controls and 29 matched colorectal cancer patients for 2-3 weeks after operation. The expression of lncRNA PVT1 was detected by quantitative real time PCR（ qRTPCR） in all samples and 29 patients with colorectal cancer were compared before and after operation. The correlation between plasma PVT1 expression and clinicopathological parameters of colorectal cancer was further analyzed,and receiver operating characteristic（ ROC） curve was used to evaluate the diagnostic efficiency of plasma PVT1 for colorectal cancer. Results： The expression levels of plasma PVT1 in the colorectal adenoma group,the colorectal cancer group and the relative early TNM Ⅰ/Ⅱ colorectal cancer group were all significantly higher than those in the health group（ all P〈0. 05） and decreased signifi-cantly after operation（ P〈0. 01）. And they were not correlated with the age,sex and tumor location,size,differentiation degree,TNM stage（ all P〈0. 05）. All diagnostic efficacy of the plasma PVT1 in the diagnosis of colorectal cancer were higher than conventional tumor marker carcino-embryonic antigen（ CEA）. The diagnostic value of combined detection of PVT1 and CEA for both colorectal cancer and TNM Ⅰ/Ⅱ colorectal cancer were higher than that of PVT1 and CEA alone. Conclusion： The up-regulated plasma lncRNA PVT1 expression in patients with colorectal cancer and precancerous lesions could be served as a potential circulating marker for the clinical diagnosis and postoperative monitoring of colorectal cancer.