微藻生物质两段式暗发酵强化氢气和甲烷产量的实验研究

Experiment Study on the Yield of Hydrogen and Methane by two-stage Dark Fermentation of Microalgae Biomass

微藻生物质厌氧消化生产氢气和甲烷效率低下,研究了一种新型微藻处理工艺即两段式暗发酵提高氢气和甲烷产量。结果表明微藻生物质的最佳有机负荷为10 g/L,相应的氢气产量为18.6 mL/g(每克挥发性有机质产气量)。进一步研究表明蛋白酶预处理能进一步提高水解酸化相中氢气的产量至35.5 mL/g,反应pH最低为6.0。同时,蛋白酶预处理能够提高产甲烷相中甲烷产量;并且最大产量为251 mL/g,显著高于空白对照组。机理研究表明,两段式消化分别为水解酸化相和产甲烷相提供最佳环境。

The productions of hydrogen and methane from microalgae biomass anaerobic digestion are inefficient. A novel microalgae treatment process,two-stage dark fermentation,was reported to increase the production of hydrogen and methane. The results showed that the optimum organic loading of microalgae biomass was 10 g/L and the corresponding hydrogen production was 18. 6 mL/g（ gas evolution per gram of volatile suspended solids）.Further studies showed that protease pretreatment can further increase the yield of hydrogen in the hydrolytic acidification phase to 35. 5 mL/g,and the minimum p H value of reaction was 6. 0. Meanwhile,protease pretreatment could increase methane production in methanogenic phase,and the maximum yield was 251 mL/g,which was significantly higher than that of blank. Mechanistic studies showed that two-stage digestion provided the optimal environment for hydrolytic and methanogenic phases,respectively.