HPLC法同时测定不同品牌艾条及艾柱中山奈酚、棕矢车菊素、异泽兰黄素的含量

Simultaneous Determination of Kaempferol,Jaceosidin and Eupatilin in Different Brands of Moxa and Moxa Cone by HPLC

目的:对不同品牌艾条及艾柱中主要有效黄酮的含量进行测定,为成品艾条及艾柱的质量控制提供参考。方法:采用DIONEX ULTIMATE 3000高效液相色谱仪(泵:LPG-3400SD Quaternary Analytical Pump,化学软件:CM-CHM-1:Server+1x TB1+Client+Control),紫外检测器(VWD-3100 Single Wavelength Detector),色谱柱为Agi Lent ZORBAX Extend-C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.2%磷酸溶液(50∶50),流速为0.8 ml·min-1,检测波长为360 nm,检测器柱温为30℃,以山柰酚、棕矢车菊素、异泽兰黄素为对照品,对不同品牌艾条及艾柱中山柰酚、棕矢车菊素、异泽兰黄素的含量进行比较分析。结果:11批样品均含有山柰酚、棕矢车菊素、异泽兰黄素,但含量差别较大。山柰酚、棕矢车菊素和异泽兰黄素分别在0.049~0.244μg(r=0.999 9)、0.095~0.475μg(r=0.999 3)、0.190~0.590μg(r=0.999 9)范围内,其质量和峰面积之间均呈良好的线性关系;平均加样回收率分别为100.69%,100.31%,100.14%;RSD分别为1.21%、1.24%、1.14%(n=5)。结论:该方法操作简便、准确度高、重复性好,可作为艾条及艾柱中山柰酚、棕矢车菊素和异泽兰黄素的含量测定方法,也可用于艾条及艾柱的品质评价和质量控制。

Objective： To determine the contents of main effective flavonoids in different brands of moxa and moxa cone for the quality control of finished Moxa products. Methods： An Agilent ZORBAX Extend-C18 column（ 250 mm × 4. 6 mm,5 μm） was used with the mobile phase consisting of acetonitrile-0. 2% phosphoric acid solution（ 50 ∶ 50）. The flow rate was 0. 8 ml·min-1,the detection wavelength was 360 nm,and the column temperature was 30℃. Kaempferol,jaceosidin and eupatilin as the reference substances,the contents of kaempferol,jaceosidin and eupatilin in different brands of moxa and moxa cone were comparatively analyzed.Results： Eleven batches of the samples contained kaempferol,jaceosidin and eupatilin,while the contents had significant differences.In the range of 0. 049-0. 244 μg（ r = 0. 999 9）,0. 095-0. 475 μg（ r = 0. 999 3）,0. 190-0. 590 μg（ r = 0. 999 9）,respectively,there was a good linear relationship between the mass and peak area. The average recovery was 100. 69%,100. 31% and 100. 14%respectively. RSD was 1. 21%,1. 24% and 1. 14%（ n = 5）. Conclusion： The method is simple,accurate and reproducible,which can be used for the quality evaluation and quality control of moxa products.