胃食管反流中的胃蛋白酶对食管外鳞状上皮细胞的影响

The effect of pepsin on extraesophageal squamous epithelial cells in gastroesophageal reflux

在实验室检查中,胃蛋白酶能迅速分解蛋白质,这是其化学分析的基础。然而,这种效应在食管外组织中是微妙的,胃蛋白酶在pH<7时是稳定的并可经重新酸化后恢复活性,从而持续降低细胞本身的防御和威胁其活性。利用体外细胞培养实验探究人胃蛋白酶3B(从胃液中纯化)对喉上皮细胞影响的相关研究证实了这些改变。本文就胃食管反流中的胃蛋白酶黏附于喉上皮细胞,消除其细胞防御并经胞吞作用后在细胞内造成进一步损害的发生发展过程及其可能机制进行综述,以期为研发显著减少胃蛋白酶原分泌、阻断已黏附的胃蛋白酶效应的新药治疗反流性疾病提供新的靶向。

In laboratory studies,pepsin swiftly breaks down protein,the basis of its chemical assay.However,its effect on extraesophageal tissues is subtle. Pepsin is stable up to pH 7 and regains activity after reacidiflcation. Thus, it perhaps sustained, depletes the cells of its defences and threatens its viability. These changes have been demonstrated in related studies which explored the effect of human pepsin 3 B（ purifled from gastric juice） on laryngeal epitheliumusing ex vivo systems and cell culture studies. In this paper, we review the development process and its possible mechanism that pepsin adheres to laryngeal cells, depletes its defences, and causes further damage internally after its endocytosisin gastroesophageal reflux. The development of novel drugs that dramatically reduce pepsinogen secretion, block the effect of adherent pepsin may provide a promising target for the treatment of reflux disease.