Box-Behnken响应面法优化青龙衣中胡桃醌提取工艺

Optimization of Extraction Process for Walnut Quinone from Juglans Green Peel by Box-Behnken Response Surface

目的对青龙衣中胡桃醌的提取工艺进行优化。方法在单因素试验基础上,选取料液比、超声提取温度和乙醇浓度为自变量,胡桃醌得率为响应值,根据Box-Behnken试验设计原理,采用响应面法对青龙衣中胡桃醌的提取工艺进行优化。结果胡桃醌最佳提取工艺为14.30倍量89.81%乙醇,30.28℃恒温提取。胡桃醌得率为2.034 mg/g,与实测值1.957 mg/g偏差较小。结论优选的提取工艺合理可行,可为提取青龙衣中的胡桃醌提供依据。

Objective To optimize extraction process of walnut quinone from Juglans green peel. Methods On the basis of single factor tests, taking material-liquid ratio, extracting temperature and ethanol concentration as independent variables, yield of walnut quinone in response to as a value, according to Box-Behnken experiment design principle, extraction process of walnut quinone from Juglans green peel was optimized by response surface analysis. Results The optimum extraction process of walnut quinone was： 14.30 times the amount of 89.81% ethanol; 30.28 ℃ constant temperature. The extracting amount of walnut quinone was 2.034 mg/g, which was close to the experimental results of 1.957 mg/g. Conclusion The optimized extraction process is reasonable and feasible, which can provide reference for the extraction of walnut quinone from Juglans green peel.